Molecular Biology Kit, EZ-10 Spin Column RNA Cleanup and Concentration Kit, 50 Preps

Introduction

EZ-10 Spin Column RNA Cleanup & Concentration kit is designed for rapid purification and concentration from in vitro transcription products or total RNA isolated from various methods such as labeling or DNase digestion.

Storage and Stability

Transported at room temperature. Upon receipt, store all components at 4°C. The kit is stable for up to 12 months at 4°C.

Features

• Recovery of RNAs larger than 20nt.
• No toxic organic chemicals used.
• Rapid and convenient, the whole procedure takes only 5 minutes.
• Rate of recovery higher than 80%.
• Compatible with most downstream applications.

NOTE: Care must be taken when working with RNA. It is important to maintain an RNAse-free environment starting with RNA sample preparation and continue through purification and analysis. Use RNAse free tubes, tips, gels. Wear gloves at all times.

Starting Material

A maximum of 100 µg RNA can be used in the RNA cleanup protocol. This amount corresponds to the binding capacity of the RNeasy mini columns.

Important Notes:

We recommend DNAse digestion or kit BS88133 to prepare DNAfree RNA.

*Before use, add 48 ml of 100% ethanol (RNase free) to 12 ml Universal RPE Solution.

Product Info