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qPCR for foodborne pathogen detection

Nov 27, 2019 11:11:43 PM

There are many opportunities for food contamination to take place in the complex food supply chain. SMARTCHEK was developed to help processers and suppliers do their part to help make sure their product is contamination free.

Real Time PCR (qPCR) is one of the most well known and versatile molecular testing technologies that provides high sensitivity and accuracy and is replacing older technologies such culture and ELISA based tests. SMARTCHEK ultra-fast qPCR based food safety testing kits provide high efficiency and accuracy. The typical running time of these probe based assays on the GENECHECKER UF-150 is about 30 minutes saving time, money and allows for much quicker processing and shipping. 

Using the Genechecker UF-150, tests are typically completed in about 30 minutes.   Perfect for on-site detection.

Interagency Food Safety Analytics Collaboration identified four priority pathogens that it believes were responsible for about 21% of foodborne llnesses, 56% of related hospitalizations and 54% of related deaths annually in the United States. These are Salmonella, E-Coli, Listeria monocytogenes and Campylobacter. The portfolio of SMARTCHEK foodborne pathogen detection kits cover these major foodborne pathogens.

Highly Sensitive, specific assays for the detection of major foodborne pathogens.

 

 

 

 

Posted in Technical By Southern Labware, Inc.

3D Cell Culture

Nov 24, 2019 8:27:00 PM

Good bye flat biology, hello 3D tissues in your favorite plate!  Meet Lena Biosciences’ SeedEZ 3D cell culture scaffold. Put the scaffolds into the wells of your favorite plate, add cells and medium, and voila! Cells grow in three dimensions, as in living tissues. Continue with your standard assays, or fluorescent imaging, and experience the upgrade in cell functions!

About Lena Biosciences:

Lena Biosciences develops cutting-edge cell culture products that upgrade cell biology to a physiologically relevantsetting. Their patented, 3D cell culture scaffold, SeedEZ, grows cells in three dimensions as in living tissues. SeedEZ enables cells to utilize both their cadherin (cell-cell) and integrin (cell-ECM) receptors that are vital for tissue-representative cell behavior, drug responses and cell therapy outcomes. Their patented multiwell-plate perfusion system, PerfusionPal, mimics what happens inside the living tissues. By mimicking supply of blood flow, PerfusionPal delivers and distributes nutrients and drugs to cells cultured in SeedEZ scaffolds. Lastly, their Blood Substitute carries and releases air, mimicking the function of hemoglobin, and maintains stable pH. This allows superior delivery of oxygen to cells in PerfusionPal, and increases cellular respiratory and drug metabolism 5x – 10x compared to cells in a dish.

 

Check them out here:

3D Cell Culture

Cell Culture Perfusion

Cell Migration and Invasion

 

Lena

 

 

 

 

 

Posted in New Products By Marketing

In our continued effort to offer our customers products of the highest quality and value, we are excited to annouce the JG Finneran Associates line of chromatography vials, caps and septa has been added to our website. 

JG Finneran Associates was founded in 1977 in Vineland, New Jersey and to this day an independently owned family business.  From the beginning, JG Finneran Associates has been an industry leader recognized worldwide for glass and plastic design.  
 
 
The US manufacturing operation has obtained ISO 9001:2015 for manufacturing and distribution of products.  Their factory is equipped with the most modern and customized machines capable of highly automated glass and plastic manufacturing.  Which results in the highest quality products for your chromatography needs.
 
Please shop our extensive product offering of Finneran chromatography products.  For questions about autosampler compatibility or for samples please contact sales@southernlabware.com.
 
 
 
Posted in New Products By Southern Labware, Inc.

Total Suspended Solids (TSS)

Oct 17, 2019 8:56:49 PM

One of our most valuable resources is clean water.  The most visible and largest sources of water pollution are suspended solids.  The measure of the amount of Total Suspended Solids (TSS) is a water quality measurement that identifies the suspended load carried by a body of water.

We get asked alot about the procedure and the supplies needed to perform the test.  Here is some information that may help.

 

 

 

 

Vaccum Filtration System

 

Glass Microfiber Filter

 

Aluminum Weighing Dish

 

Drying Oven

 

Desiccator

 

Analytical Balance

 

 

 

 

 

 

 

 

 

 

 

 

 

Posted in Technical By Southern Labware, Inc.

nUVaClean™ UV Pipette Carousel

Jul 18, 2019 9:51:11 PM

One of our core principals at Southern Labware is to partner with manufacturers who create innovative new products.  It is exciting for us to offer our customers new products that are not only functional but can improve the quality of their research.  MTC Bio is one of those manufacturers and the nUVaClean UV Pipette Carousel is one of those products.  We have been waiting months for this product to launch and we are pleased to introduce this product to you.

MTC’s ProPette nUVaClean™ is the first and only pipette rack that not only organizes and protects six pipettes, but also destroys up to 99% of unwanted contamination. The new nUVaClean™ uses a germicidal UV-C mercury vapor lamp. A high-efficiency reflector/concentrator completely bathes pipette shafts in 254nm UV light. This exposure destroys unwanted microbiological contaminants and prevents cross-contamination in PCR and other sensitive procedures.

Just place single-channel pipettes of any range or brand in the nUVaClean™ and press the “start” button to begin the auto-decon cycle. After 28 minutes the nUVaClean will power down automatically, delivering up to 6 pipettes that are safe, clean and ready for use.

Features:

  • UV-C Lamp and reflector system destroys 99% of DNA
  • Eliminates cross-contamination
  • 360° UV Exposure
  • Fits virtually any pipette
  • Compact 6.5in. (17cm) footprint
  • Made in the USA

 

 

 

 

BUY nUVAClean Now!

 

 

Posted in New Products By Southern Labware, Inc.

Is a wooden flammable storage cabinet counter intuitive?   We thought so until we partnered with SciMatCo, a manufacturer of hazardous materials storage cabinets.  Here is what we learned about the benefits of a wooden flammable storage cabinet.

 
Wood burns.  So how can it possibly better than a metal cabinet in a fire.  First, wood is a thermal insulator and will not distort or bend in the heat of a fire. The rapid rise in temperature due to a fire will have minimum impact on the inside temperature of a fire.  Metal conducts heat.  During a fire metal flammables cabinets can and will pass the rapid rise in temperature on to the contents in the flammables cabinets.  Glass bottles in a metal flammables cabinet have been know to break, releasing their vapors and liquids.  Metal containers can pop their lids due to high temperatures experienced during a fire.  In short, metal cabinets become ovens when involved in a fire.
 
Another key point to consider is that wooden flammable cabinets do not distort or bend when in a fire.  Metal flammable cabinets can bend and distort in a fire, opening themselves and their contents to the flames.  In order for the fire to get inside a wooden cabinet is for it to burn all the way thru.
 
Each flammables cabinet is constructed with 1" plywood using specifications developed by both OSHA, NFPA and the UFC to meet all the requirements for a flammables cabinet.
 
 
  • Meets all OSHA, NFPA (National Fire Protection Association) and UFC (Uniform Fire Code) standards
  • Cabinets are constructed of 1" thick, multi-ply, exterior-grade plywood
  • Finished with yellow chemical-resistant paint (inside and out)
  • Provided with lock and two keys; all hardware chemical-resistant
  • Continuous piano-type hinges on door
  • All cabinet joints are rabbeted to provide exceptional cabinet strength
  • Cabinet floor constructed as a 2" liquid-tight trough to contain spillage 
  • Self-closing doors available upon request

 

Please watch the short video of a wooden SciMatCo flammables cabinet and a metal flammables cabinet both engulfed in flames for 12 minutes.  We were just as surpised at the results as you will be.

 

 

 

 Click here to check out our Newest Product Addition Scimatco

Posted in New Products By Southern Labware, Inc.

Southern Labware now accepts Apple Pay

Dec 27, 2018 10:08:00 PM

Southern Labware now accepts Apple Pay as a more convenient and secure way for our customers to shop.

Shopping with Apple Pay is as simple as the touch of a finger with Touch ID, ending the frustration of repeatedly filling out shipping and billing information and keeping tracking of credit and debit cards.

More importantly security and privacy is at the core of Apple Pay.  When you use a credit or debit card with Apple Pay, the actual card numbers are not stored on the device, nor on Apple servers. Instead, a unique Device Account Number is assigned, encrypted and securely stored in the Secure Element on your device. Each transaction is authorized with a one-time unique dynamic security code.

Simply click the Apple Pay button at checkout and experience the Apple Pay difference!

 

Apple Pay

Posted in News By Marketing

Bio Basic Inc. Life Science Products

Dec 22, 2018 10:08:41 PM

In our continued effort to offer premium products that add value, quality and reliability to your lab, we are pleased to offer Bio Basic Inc. life science products. 

Bio Basic Inc. is a life science products manufacturer founded in Toronto, Canada with Headquarters in Markham, Ontario and Amherst, New York.  
 
Our relationship with Bio Basic Inc. will allow us to offer molecular biology kits, RNA and DNA modifiying enzymes and DNA, RNA and protein markers and vectors.
 
 
Biobasic 
Posted in New Products By Marketing
 A researcher once said to me, "Does the world really need another centrifuge?"  With the countless brands flooding the pages of catalogs and websites it was hard to argue his point.   

Until now!  Heathrow Scientific has developed a dual purpose centrifuge/magnetic stirrer called the MagFuge.  The MagFuge's patented dual design provides magnetic stirring and high speed centrifugation in one powerful unit.  This is truly an innovative design that provides substantial cost and benchtop space savings by having two pieces of equipment in one.

Please review the Magfuge's feature and specifications below to see just how innovative it is.

The MagFuge® by Heathrow Scientific is the industry’s first high-speed centrifuge and magnetic stirrer, combined into one unit. This design innovation enables a substantial cost savings for labs by not having to purchase two pieces of equipment, a stirrer and a centrifuge, because the MagFuge® does it all.

Features:

  • Made from a durable chemically resistant ABS and polycarbonate making it easy to clean, maintenance free and helps lower the cost of ownership
  • The modern low-profile design is compact and has a small footprint for labs with limited bench space and limited height that ultimately helps reduced clutter in the lab
  • An easy to use back-lit digital control panel for time, speed and operations ensures effective outcomes and streamlines the work flow process by allowing for quick set up
  • US Design Patent No. D814,045 and Utility Patent Pending
  • Community Design No. 003515337-0001-0002 European Patent App. 3 246 088

 

High-Speed Mini Centrifuge

The high-speed Centrifuge (12,500 RPM) is designed to give you maximum speed and flexibility while being comfortable and easy to use.

  • Ideal for most protocols requiring fast spins (12,500rpm / 9,800 x g), and appropriate for PCR, microfilter cell separation, and HPLC protocols
  • Adjustable time (30 seconds to 60 minutes) and speed (500 to 12,500 RPM)
  • Variable speed can be set in RPM or RCF and has rapid acceleration and fast controlled breaking
  • Two rotors included, 12 place for 1.5/2.0 mL tubes and 6 place for 5 mL tubes with adapters for additional tube sizes
  • Manual lid override allows users to open lid in case of a power failure and prevents loss of samples
  • Safety features help ensure it will not operate when the lid is open, a rotor is jammed, or the unit is out of balance

 

 magfuge

 

magfuge

 

Magnetic Stirrer

The high-performance Magnetic Stirrer (3L Plus) is designed to provide a powerful magnetic coupling that ensures consistent accurate alignment of the stir bar that significantly reduce the chance of spin-outs.

  • Ideal for work with sensitive samples such as cell lines and protocols requiring more aggressive mixing to quickly achieve full dilution of samples in solution
  • Multiple stirring modes, clockwise, c. clockwise and oscillate enable protocols to be controlled precisely for reproducible results
  • Adjustable time (30 seconds to 60 minutes or continuous) and speed (50 to 2,500 RPM)
  • After hours of continuous use, the unit remains cool ensuring sample integrity
  • Sensor technology prevents the stirrer rotor from being used above 2,500 RPM helping to protect users and prevent unit damage
  • Two silicone mats are included to help prevent spills and to help keep vessels stable
  • High-quality rare-earth magnets are used in the stirrer rotor to ensure the strongest magnetic coupling
  • Two rare-earth stir bars (40 x 12 mm) included have PTFE coating for chemical resistance

 

magfuge

 

Precision, literally at the touch of one button!

The MagFuge® offers an easy to program digital display.

Multiple functions and options are available to achieve the best possible centrifugation and stirring results for a wide range of protocols.

 

magfuge

 

Conveniently rotate the knob to choose the function desired (centrifuge or stirrer) and push to lock in the selection

After selecting a function, rotate through the performance options and select the program to meet your requirements.

  • Speed Display or Stirrer Mode
  • Speed: Centrifuge 500 to 12,500 RPM Stirrer 50 to 2,500 RPM, 5 sec. increments
  • Time: 30 seconds to 60 minutes, 5 sec. increments (continuous option for stirrer)

 

magfuge

Last run memory feature is avaliable for repeat protocol requirements.

 

 

Technical specifications:

Model Number 120582
Power Universal 12V DC input 4 cord sets: Low voltage, double insulated power adapter
Weight  1.85 kg (4.08 lb)
Certifications CE, SGS, RoHS2, WEEE
Operating Temp Range 2°C to 40°C
Max Relative Humidity  Non-condensing, 80% for temperatures up to 31°C decreasing linearly to 50% R.H. at 40°C
Warranty 3 Years
Dimensions (L x W x H) 9.2 x 7.3 x 4.7 in (23.5 x 18.5 x12 cm)
Color Gray/Blue
MagFuge® Centrifuge 
Speed Range 500 – 999 rpm (increments of 1); 1,000 – 5,000 rpm (increments of 5); 5,000 – 12,500 rpm (increments of 25)
RCF 16 – 9,783 x g (max RCF calculated)
Capacity 12 place 1.5/2.0 mL tube rotor: 24 mL total maximum liquid volume
6 place 5 mL tube rotor: 30 mL total maximum liquid volume
Run Time Ranges 30 seconds – 60 minutes (5 second increments)
MagFuge® Magnetic Stirrer 
Speed Range 50 – 999 rpm (increments of 1); 1,000 – 2,500 rpm (increments of 5)
Capacity 3L total maximum vessel liquid volume. Diameter of vessel not to exceed 130 mm (6.70”)
Run Time Ranges 30 seconds to 60 minutes or continuous (5 second increments)
Oscillating Period 1 second – 60 minutes or continuous
Stir Bar Dimensions 40 x 12 mm cylindrical shape, rare-earth 
Plate Dimensions Approx 130 mm diameter

 

Package Includes:

  • MagFuge®
  • Three Rotors: 5 mL Tube Rotor, 1.5/2.0 mL Tube Rotor, Stirrer Rotor
  • 12 Tube adapters 0.2 mL
  • 12 Tube adapters for 0.5 mL
  • 2 Silcone mats for stirrer lid
  • 2 Rare-Earth PTFE coated stir bars stir bars
  • 1 Low voltage, double insulated power adapter with 4 cord sets

 

Purchase a Magfuge!

 

 

Posted in New Products By Southern Labware

A guide to microbead milling.

Aug 2, 2017 9:40:35 PM

What is microbead milling? Microbead milling is a homogenization process used to break up (lyse) samples in order to release the DNA, RNA and proteins contained within the cells. Samples are placed in tubes with the appropriate grinding beads and subjected to high energy mixing. The beads impact the sample, eventually breaking it down on the cellular level releasing subcellular contents. The samples are then typically centrifuged and the lysate recovered from above the beads.

 
The Benchmark BeadBug™,  BeadBug™ 6 and BeadBlaster™ 24 are bead beating homogenizers, designed to rapidly homogenize small samples in disposable tubes with the aid of grinding beads. Both instruments work by physically moving tubes, samples, and grinding beads in an oscillating motion several thousand times per minute. Through this motion, the beads tear into and crush samples on impact. They are different in capacity - if only a few samples require processing a day, then the BeadBug will be sufficient for most researchers. For those with higher capacity demands or a larger lab group, the BeadBlaster 24 is more practical. 
 
Microbead homogenizing can be effective at disrupting many different sample types. The key aspect of successfully homogenizing samples is to match the correct bead to the sample and then not to overload the disruption tube. For bead beating to be effective, the beads need to move and crash into the sample. Tubes packed with solid tissues and/or filled with buffer prevent the beads from moving freely. 
 
General guidelines that can help to ensure that samples are homogenized effectively include: 
 
•Do not over fill tubes. The total volume of the sample, beads, and buffer should never exceed half the volume of the tube. Less is better. 
•Sample and buffer should be twice the volume of the beads. 
•Detergents should be kept to a minimum as the foaming will impede the movement of the beads. 
•Solid tissues should never be more than 1/20th the volume of the disruption tube, i.e., no more than 100 mg tissue in a 2 ml tube. 
•If sample warming is an issue, process the sample in short bursts. 
 
A variety of prefilled tubes are available for homogenizing samples including those with glass and Triple-Pure™ zirconium beads. Both bead types are acid washed and processed to remove nucleases and proteases. The Triple-Pure bead tubes undergo special handling during filling and are quality tested to ensure the elimination of DNase, RNase, protease, and nucleic acids. When samples are used for molecular biology applications, the high purity of the Triple-Pure beads is preferred.
 
Both glass (silica) and zirconium beads are effective at disrupting cells and tissues. The added density of zirconium helps to yield better homogenized samples. Benchmark has nine varieties of prefilled tubes, each which can be used with a general category of sample. These tubes are: 
 
Standard Glass Beads, 0.1 mm – Effective for the homogenization of bacteria. Best used for cultured microbes where low cell densities are not an issue. 
 
Standard Glass Beads, 0.5 mm – For larger microbial cells, like yeast. Effective for homogenizing yeast cultures, especially where heat labile proteins might be involved. 
 
Standard Glass Beads 1.0 mm – For small pieces of soft tissue, fungal mycelium, and larger algal cells. 
 
Triple-Pure Zirconium Beads, 0.1 mm – For all bacteria and low density cultures. 
 
Triple-Pure Zirconium Beads, 0.5 mm –Effective for the homogenization of yeast and smaller algae cells. Effective for low density cultures and samples where contamination is an issue. 
 
Triple-Pure Zirconium Beads, 1.0 mm – Use for fungal mycelium, soft tissues, very small tissue samples. 
 
Triple-Pure Zirconium Beads, 1.5 mm – Effective for homogenizing softer animal tissues (adipose, mouse intestine, hypothalamus, and liver) as well as leaf and soft vegetables (e.g., potato). 
 
Triple-Pure Zirconium Beads, 3.0 mm – Provides greater energy for shearing and rupturing tough samples such as muscle, lung, and kidney as well as small plant seeds, stems, and roots.
 
Stainless Steel Beads, 2.8 mm – Stainless steel has greater density than both glass and zirconium and can be used on very tough samples such as cornea and connective tissue and in situations where the other tube types are not effective. Metals beads are effective at homogenizing tough samples, but they also generate significant heat. Care should be taken when working with heat labile molecules. Plant extracts will often oxide rapidly in the presence of stainless steel.
 
Sample Types:
 
Bacteria – As bacteria tend to be very small, 1-3 µm in length or diameter, smaller beads normally work better for cell disruption. A culture of bacteria contains upwards of 109 cells/ml thus smaller beads (e.g., 0.1 mm) allow for greater surface area for impacting cells. This is a very significant factor in cracking bacteria. Generally the mass of the beads greatly exceeds the mass of the bacteria, thus both glass and zirconium work well. However, glass beads tend to clump more than denser zirconium beads which settle more effectively in lysates after processing. Other organisms of comparable size, such as cyanobacteria, can also be processed in a similar manner. 
 
Yeast – The next larger microorganism after bacteria is yeast, being roughly 10+ microns in diameter or length. Beads with a diameter of 0.5 mm tend to work very well on yeast cells. Many single cell algae can be processed as if they are yeast. 
 
Filamentous Fungi – Fungi that are not solid bodies, such as pycnidia and similar fruiting bodies, are effectively disrupted with the next size of beads. Beads of 1.0 mm are very effective for shearing mycelium.
 
Plant Leaves – Leaves can be homogenized readily using 1.5 and 3.0 mm zirconium beads and 2.8 mm stainless steel beads. Leaves are often harvested by cutting out the sample with a paper hole punch. A sample of this size is normally 10-20 mg in mass, thus multiple punches can be processed in one tube. 
 
Shoots and Stalks – More resilient plant material can be effectively homogenized using the larger bead sizes. Stalks of bundled grasses and young roots and shoots can be effectively homogenized using these beads, however plants which are more mature may have significant pulp following homogenization if their lignocelluloses content is high. Sample size should not exceed 50-70 mg to ensure good disruption. 
 
Seeds – Small fresh seeds can be processed effectively using 3.0 mm zirconium and 2.8 mm stainless steel beads. Larger seeds must first be dissected so that the sample mass is no more the 50 mg. 
 
Soft Animal Tissue – Animal tissues can be grouped into soft and hard tissue types. The softer tissues, such as liver, adipose, and hypothalamus, can readily be disrupted using 1.5 mm beads or larger. Though easily homogenized, it is still important not to overload tubes with soft animal tissues. 
 
Resilient Animal Tissues – Fibrous animal tissues such as muscle, lung, kidney and heart can be difficult to homogenize. By using larger beads, these tissues can be effectively disrupted to liberate RNA, DNA and proteins with very high yields. Triple-Pure 3.0 mm zirconium beads and stainless steel beads (2.8 mm) are effective for disrupting these tough tissues. Sample size is best kept under 50 mg while buffer should be no more than 200-300 µl. Many RNA extraction protocols use larger volumes of buffer, and for these processes the sample can be homogenized in less extraction buffer and then brought up to volume following homogenization.
 
 
bead kit
 
Posted in Technical By Benchmark Scientific
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