One-Step RT-PCR Kit
The Accuris One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. Formulated for easy, one tube production of cDNA and subsequent end point PCR of any RNA template, the kit is highly sensitive, and can be used with as little as 1pg of total RNA for the starting material.
A blend of a highly thermostable reverse transcriptase (45° - 55°C), Accuris Hot Start Taq Polymerase and a potent RNase inhibitor in an optimized buffer allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). Inclusion of a hot start polymerase allows for reverse transcription to take place before the polymerase is activated, resulting in higher specificity. It also offers the convenience of reactions being set up at room temperature.