Molecular Biology Kit, EZ-10 Spin Column Total RNA Miniprep Kit, 50 Preps
Molecular Biology Kits
Introduction
The EZ10 Column Total RNA MiniPrep Kit allows efficient purification of total RNA from various samples, total RNA is easily purified from animal or human cells and issues using a simple spin format. This kit simplifies total RNA isolation by combining the stringency of guanidine isothiocyanate lysis with the speed and purity of silicabased purification.
Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide optimal binding conditions. The lysate is then loaded onto the EZ10 column with a silica membrane. RNA binds to the silica membrane, all protein and other components are removed in the flowthrough. Remaining contaminants and salts are efficiently washed away. Purified RNA is eluted in RNasefree water has OD260/OD280 ratios of 1.92.1 (measured in 10 mM TrisHCl, pH 7.5) and is ideal for use in most downstream applications including Northern blotting, RTPCR, Quantitative PCR, Poly (A) RNA selection and Array analysis.
Components | BS1361 |
Amount | 50 Preps |
Buffer RLT | 25 ml |
Buffer RW | 30 ML |
Universal RPE Solution | 12 ml |
Rnase-free Water | 5 ml |
EZ-10 Spin Column | 50 |
2 ml Collection Tube | 50 |
Protocol | 1 |
Important Notes:
Universal RPE Solution is supplied in a concentrated form, before use, add 48ml 96100% ethanol to 12 ml concentrated universal RPE solution and mix well.
Care must be taken when working with RNA. It is important to maintain an RNasefree environment starting with RNA sample preparation and continue through.
Part# | BS1361 |
Decription | EZ-10 Spin Column Total RNA Miniprep Kit |
Sterile | No |
Storage Conditions | 18 to 25C |
Qty | 1 |
Shipping Conditions | RT |
Size | 50 Preps |
Technical Specifications |
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