Molecular Biology Kit, EZ-10 Spin Column Plant RNA Miniprep Kit, 50 Preps

Introduction

Polysaccharides and polyphenols are components of plants, It is very difficult to remove after form insoluble compounds closely combining with RNA. EZ Spin Column Plant total RNA Purification Kit is applicable to all kinds of plant samples RNA rapid extraction. Cracking liquid can effectively solve the difficult problem such as polyphenols easy oxidation, polysaccharide separation and nucleic acids compounds. RNA Purification using spin column is easy to operate, avoid ethanol rinse. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection and in vitro translation.

Features:

• Fast. Using a rapid spin-column format, the entire procedure takes approx 30 minutes.
• Versatile. Suitable for isolation of RNA from a wide range of specimens such as arabidopsis thaliana, tobacco, camphor and other samples.
• High Quality of RNA. Complete removal of contaminants such as genomic DNA, polysaccharides, polyphenols and other impurities. An OD260/OD280 ratio of purified RNA is generally > 1.9.

NOTE:  Care must be taken when working with RNA. It is important to maintain an RNAse-free environment starting with RNA sample preparation and continue through purification and analysis. Use RNAse free tubes, tips, gels. Wear gloves at all the time.

Materials Supplied by User:

• Microcentrifuge capable of at least 12,000 × g
• RNase-Free pipets and pipet tips
• Vortexer
• RNase-Free Ethanol (96-100%)
• RNase-Free Microcentrifuge tubes (1.5 ml or 2 ml)

Note:

Universal GT Solution and Universal NT Solution are supplied in a concentrated form, before use; add 12 ml 96-100% ethanol to 18 ml concentrated universal GT solution and 24 ml 96-100% ethanol to 6 ml concentrated universal NT solution to make a work solution.

Storage

The kit is valid for 1 year at 4°C. Transportation at room temperature, store at 4°C.

Product Info