Molecular Biology Kit, EZ-10 Spin Column Total RNA Miniprep Super Kit, 250 Preps

Storage:

The kit is stable for 18 months at room temperature.  For longer storage, keep all contents cold.

Kit Contents:

RLT Solution should be kept at 2-8oC. It may form a precipitate upon storage. If necessary, dissolve the precipitate by warming the solution at 370C.

Before use, add 20 ml of 100% of ethanol to 5 ml RPE Solution for BS583, 88 ml of 100% ethanol to 22 ml RPE Solution for BS584, or 120 ml of 100% ethanol to 30 ml RPE Solution for BS784. For other volumes of RPE Solution, simply add enough ethanol to make a 4:1 ratio (volume of added ethanol: volume of RPE Solution = 4:1)

Principle

This kit is designed for fast isolation of total RNA from bacteria, yeast, fungi, plant and animal tissues.  The kit contains a membrane embedded spin column for binding up to 10 µg of RNA.  Nucleotides, proteins, salts, and other impurities do not bind to the EZ-10 Column.

Applications:

• Preparation of Total RNA from Various sources.
• RT-PCR
• Differential display
• cDNA synthesis
• PolyA + RNA selection 
• RNase/S1 nuclease protection

Features:

• Preparation of high quality total RNA from animal cells or other sources.
• Rapid and Economical: entire procedure takes about 20 minutes (Note: timing for preparation of samples is not included).
• High yield
• No phenol/chloroform extraction or ethanol percipitation needed

Note:  Care must be taken when working with RNA.  It is important to maintain an RNAse-free environment starting with RNA sample preparation and continue through purification and analysis.  Use RNAse free tubes, tips, gels.  Wear gloves at all the time.