Molecular Biology Kit, EZ-10 Spin Column Total RNA Miniprep Super Kit, 100 Preps
Molecular Biology Kits
Storage:
The kit is stable for 18 months at room temperature. For longer storage, keep all contents cold.
Kit Contents:
Components | BS583 | BS584 | BS784 |
Amount | 50 Preps | 100 Preps | 250 Preps |
RLT Soulution* | 14 ml | 70 ml | 175 ml |
RW Solution | 12 ML | 60 ml | 150 ml |
RPE Solution* | 5 ml | 22 ml | 2 x 30 ml |
Rnase-free Water | 1 ml | 5 ml | 12.5 ml |
EZ-10 Spin Column & 2 ml Collection Tube | 20 | 100 | 250 |
Protocol | 1 | 1 | 1 |
RLT Solution should be kept at 2-8oC. It may form a precipitate upon storage. If necessary, dissolve the precipitate by warming the solution at 370C.
Before use, add 20 ml of 100% of ethanol to 5 ml RPE Solution for BS583, 88 ml of 100% ethanol to 22 ml RPE Solution for BS584, or 120 ml of 100% ethanol to 30 ml RPE Solution for BS784. For other volumes of RPE Solution, simply add enough ethanol to make a 4:1 ratio (volume of added ethanol: volume of RPE Solution = 4:1)
Principle
This kit is designed for fast isolation of total RNA from bacteria, yeast, fungi, plant and animal tissues. The kit contains a membrane embedded spin column for binding up to 10 µg of RNA. Nucleotides, proteins, salts, and other impurities do not bind to the EZ-10 Column.
Applications:
- Preparation of Total RNA from Various sources.
- RT-PCR
- Differential display
- cDNA synthesis
- PolyA + RNA selection
- RNase/S1 nuclease protection
Features:
- Preparation of high quality total RNA from animal cells or other sources.
- Rapid and Economical: entire procedure takes about 20 minutes (Note: timing for preparation of samples is not included).
- High yield
- No phenol/chloroform extraction or ethanol percipitation needed
Note: Care must be taken when working with RNA. It is important to maintain an RNAse-free environment starting with RNA sample preparation and continue through purification and analysis. Use RNAse free tubes, tips, gels. Wear gloves at all the time.
Part# | BS584 |
Decription | EZ-10 Spin Column Total RNA Miniprep Super Kit |
Sterile | No |
Storage Conditions | 18 to 25C |
Qty | 1 |
Shipping Conditions | RT |
Size | 100 Preps |
Technical Specifications |
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