Benchmark Scientific

Enhancing Scientific Endeavors: A Comprehensive Guide to Biology and Science Lab Equipment

By Benchmark Scientific
Apr 4, 2024

Enhancing Scientific Endeavors: A Comprehensive Guide to Biology and Science Lab Equipment

In the realm of scientific exploration, the significance of reliable laboratory equipment cannot be overstated. Whether you're delving into the intricate mechanisms of biology or unraveling the mysteries of chemistry, having access to high-quality lab equipment is paramount. Southern Labware stands as a beacon of excellence in providing top-tier biology and science lab equipment, empowering researchers, educators, and professionals to push the boundaries of knowledge. Let's embark on a journey through the essential equipment that forms the backbone of scientific discovery.

Autoclaves & Sterilizers:

Starting with the cornerstone of laboratory hygiene, autoclaves and sterilizers ensure the purity of experiments by effectively eliminating microorganisms. From sterilizing glassware to decontaminating surgical instruments, these devices uphold the highest standards of cleanliness, safeguarding the integrity of research outcomes.

Benchtop Centrifuges:

In the whirlwind of scientific experimentation, benchtop centrifuges emerge as indispensable tools. By harnessing centrifugal force, these devices separate substances based on density, facilitating tasks such as cell isolation, protein purification, and DNA extraction with precision and efficiency.

Blenders:

Blending the boundaries between biological and chemical sciences, blenders play a crucial role in homogenizing samples and preparing solutions. Whether it's creating cell lysates for protein analysis or concocting homogeneous mixtures for chemical reactions, blenders ensure uniformity and consistency in experimental procedures.

Cell Lysis:

Unlocking the secrets held within cells requires specialized techniques, and cell lysis stands at the forefront of this endeavor. By rupturing cell membranes, these instruments release cellular contents, enabling the extraction of DNA, RNA, proteins, and other biomolecules for further analysis and manipulation.

Heating Mantles and Hotplates:

Heating mantles and hotplates provide a controlled environment for heating substances, essential for a myriad of laboratory operations. From gentle evaporation to vigorous reflux, these devices offer precise temperature regulation, facilitating reactions, and transformations in chemistry and biology alike.

Hotplate Stirrers:

Combining the functions of heating and stirring, hotplate stirrers offer versatility in laboratory applications. Whether it's dissolving solutes in a solution or maintaining homogeneity during a reaction, these devices streamline processes and enhance experimental reproducibility.

Laboratory Glassware Washer:

Ensuring cleanliness and repeatability, laboratory glassware washers automate the laborious task of cleaning glassware. With customizable cycles and thorough cleaning mechanisms, these appliances uphold the highest standards of hygiene, minimizing contamination risks in experiments.

Magnetic Stirrers:

Harnessing the power of magnetic fields, magnetic stirrers agitate solutions with precision and control. By eliminating the need for manual stirring, these devices promote consistency in mixing and allow researchers to focus on other aspects of their experiments.

Microplate Reader:

At the intersection of biology and technology, microplate readers enable high-throughput analysis of biological samples. From measuring enzyme activity to quantifying biomolecular interactions, these instruments provide invaluable insights into cellular processes and biochemical pathways.

Orbital Shakers:

For applications requiring gentle agitation or vigorous mixing, orbital shakers offer a versatile solution. Whether it's culturing cells, staining tissues, or performing chemical assays, these devices provide uniform agitation, ensuring optimal conditions for experimentation.

Ovens:

From drying glassware to incubating cultures, lab ovens play a vital role in laboratory workflows. With precise temperature control and uniform heat distribution, these appliances provide a stable environment for a wide range of biological and chemical processes.

Overhead Stirrers:

When dealing with high-viscosity fluids or large volumes, overhead stirrers rise to the occasion. By providing powerful agitation from above, these devices ensure thorough mixing and homogenization, even in the most challenging experimental conditions.

Paddle Blender:

In the quest for sample homogenization, paddle blenders offer a robust solution. Whether it's preparing food samples for microbiological analysis or blending tissue samples for DNA extraction, these devices excel in achieving uniformity and consistency.

qPCR Machine:

At the forefront of molecular biology research, qPCR machines enable the precise quantification of nucleic acids. By combining polymerase chain reaction (PCR) with real-time detection, these instruments empower researchers to unravel the intricacies of gene expression, mutation analysis, and pathogen detection.

Thermal Cyclers:

Facilitating the amplification of DNA sequences through PCR, thermal cyclers are indispensable tools in molecular biology laboratories. By precisely controlling temperature cycles, these instruments enable the rapid and accurate replication of DNA, essential for a myriad of applications.

Thermal Mixers:

Combining heating, cooling, and agitation, thermal mixers provide a versatile platform for a range of laboratory protocols. Whether it's enzyme reactions, DNA hybridization, or protein denaturation, these devices offer precise control over temperature and mixing parameters.

Tube Rollers and Rotators:

For gentle mixing and agitation of tubes and vials, tube rollers and tube rotators offer a convenient solution. Whether it's culturing cells, hybridizing nucleic acids, or staining tissues, these devices provide consistent and uniform motion, ensuring optimal experimental conditions.

Ultra-Low Freezers:

Preserving valuable biological samples and reagents requires ultra-low freezers capable of maintaining temperatures as low as -80°C. By providing a stable and ultra-cold environment, these freezers safeguard the integrity of biological materials, enabling long-term storage and experimentation.

Vortex Mixers:

When rapid mixing or agitation is required, vortex mixers deliver swift and efficient results. Whether it's resuspending cells, mixing reagents, or dispersing nanoparticles, these devices offer versatility and convenience in laboratory workflows.

Vacuum Ovens:

For applications requiring precise control over temperature and pressure, vacuum ovens offer an ideal solution. Whether it's drying delicate samples or conducting solvent removal under reduced pressure, these ovens provide a controlled environment for sensitive materials.

Vacuum Pumps:

From filtration to distillation, vacuum pumps are indispensable tools in laboratory operations. By creating and maintaining vacuum levels, these pumps enable a wide range of applications, including solvent evaporation, degassing, and sample concentration.

Water Baths:

Providing a stable temperature environment for incubation, heating, and thawing, water baths are essential equipment in laboratories worldwide. Whether it's maintaining physiological conditions for biological samples or conducting temperature-sensitive reactions, these baths offer precise control and uniform heating.

Freeze Dryers:

Preserving the integrity of sensitive materials through lyophilization, freeze dryers remove moisture under vacuum conditions. Whether it's preserving pharmaceuticals, food samples, or biological specimens, these dryers offer a gentle and efficient method of dehydration, ensuring long-term stability.

In conclusion, the world of scientific discovery relies heavily on the tools and equipment at our disposal. Southern Labware's comprehensive range of biology and science lab equipment embodies reliability, precision, and innovation, empowering researchers to push the boundaries of knowledge and usher in a new era of scientific breakthroughs. With each experiment, each discovery, these instruments pave the way for a better understanding of the natural world and hold the promise of a brighter future for all.

Unveiling the MC-24R The Pinnacle of Refrigerated Centrifugation Excellence for Modern Molecular Labs

By Benchmark Scientific
Nov 7, 2023

Introducing the MC-24R™ Refrigerated High-Speed Microcentrifuge: A Game-Changer for Molecular Biology Labs

Welcome to the future of centrifugation with the launch of Benchmark’s flagship product – the MC-24R™ Refrigerated High-Speed Microcentrifuge, equipped with the innovative Combi-Rotor™. Tailored for the modern molecular biology laboratory, this piece of equipment is more than just a machine; it’s the partner you never knew you needed.

At the heart of the MC-24R™ lies the next-generation touch control panel, designed for intuitive operation and efficiency. With a spin capable of reaching up to 13,500 rpm (that’s an impressive 16,800 xg), it sets a new standard for high-speed centrifugation. But that’s not all – the unique COMBI-Rotor™ feature accepts both microtubes and PCR tubes/strips, streamlining workflows by obviating the need for multiple rotors.

The MC-24R™ doesn't stop there. It boasts an optional high-speed spin column rotor that can achieve 17,157 xg, a hermetically sealed rotor for contamination prevention, and a four-place PCR strip rotor for versatility.

Adjustable acceleration and deceleration rates, real-time rpm/rcf conversion, program storage of up to 99 protocols, and a status indicator light are only some of the features that make the MC-24R™ stand out. This light is an at-a-glance indicator of the unit’s status, letting you know when it’s running, in standby, or if a run is complete.

With powerful refrigeration capabilities that go as low as -20°C, the MC-24R™ is designed to handle sensitive samples that require precise temperature control.

The compact design of the MC-24R™ belies its robust functionality, making it a space-saving yet indispensable tool in any lab.

Technical prowess is matched by user-friendly features, such as:

A COMBI-Rotor™ that accommodates various tube types and sizes, lid included.
Touchscreen operation for seamless navigation.
A capacity that includes 24 microtubes, 4 PCR Strips, or 24 spin columns.
Variable temperature control for sensitive sample handling.

The MC-24R™ is not just a centrifuge; it's a commitment to quality and efficiency. It comes with a 2-year warranty and the reliability that Benchmark is known for. This refrigerated microcentrifuge is a true powerhouse, ready to take on the demands of any high-throughput laboratory.

Elevate your lab’s capabilities with the MC-24R™ Refrigerated High-Speed Microcentrifuge. It's more than just a centrifuge – it's a leap forward in laboratory innovation. Click Here to take advantage of this amazing Black Friday Sale

Posted in New Products

Innovating Lab Excellence Introducing Large Volume Screw Cap Centrifuge Tubes - Convenience, Sterility, Rewards, and Savings Await!

By Benchmark Scientific
Aug 30, 2023

Revolutionize Your Lab Experience with Our New Large Volume Screw Cap Centrifuge Tubes: Introducing Convenience, Sterility, Rewards, and Savings!

At Southern Labware, we are dedicated to supporting your scientific endeavors by providing innovative solutions that streamline your research processes and enhance your outcomes. It is with immense excitement that we introduce our latest breakthrough – the Large Volume Screw Cap Centrifuge Tubes. These tubes are meticulously designed to meet the evolving needs of modern laboratories, and they bring a host of features that will transform the way you work.

Discover Unmatched Versatility: A Range of Sizes for Every Project

We understand that no two experiments are the same. That's why we offer our Large Volume Screw Cap Centrifuge Tubes in a variety of sizes: 175ml, 225ml, 250ml, and 500ml. Whether you're running small-scale assays or conducting large-scale experiments, these tubes provide the flexibility and capacity you require for accurate and reliable results.

Innovative Screw Cap Design: Efficiency Meets Security

Bid farewell to inconvenient closures and the anxiety of leaks during centrifugation. Our centrifuge tubes feature a user-centric screw cap design that guarantees a secure seal, safeguarding your samples and preventing contamination even at high speeds. With this revolutionary design, you can focus on your research without compromising the integrity of your valuable specimens.

Bulk Packaging: Seamlessness at Its Best

Time is of the essence in a bustling lab environment. We've got you covered with our bulk packaging option for the Large Volume Screw Cap Centrifuge Tubes. Say goodbye to repetitive unwrapping and experience the convenience of uninterrupted workflow. This option is perfect for those who need larger quantities of tubes without the hassle of individual packaging.

Sterile Assurance: Confidence in Your Results

Your research is only as reliable as your samples. Our centrifuge tubes are manufactured under stringent conditions and undergo rigorous sterilization processes to ensure that your experiments remain contamination-free. This commitment to sterility not only enhances the accuracy of your findings but also contributes to the reproducibility of your work.

Double Rewards Points: Appreciating Your Loyalty

We value your continued support and trust in our products. As a token of our gratitude, we're excited to introduce double rewards points for every purchase of our Large Volume Screw Cap Centrifuge Tubes. Your loyalty deserves to be rewarded, and we're delighted to make your commitment to us doubly worthwhile. Enjoy a range of exclusive benefits by redeeming your accumulated points.

Save 10% with the Labware Essentials Subscription Service (LESS)

In our ongoing effort to enhance your experience, we're thrilled to offer the Labware Essentials Subscription Service (LESS). When you sign up, you not only secure a steady supply of our innovative centrifuge tubes but also enjoy a 10% discount on each shipment. This subscription is tailored to your needs, ensuring that you have the tools you require while saving time and money.

In conclusion, the Large Volume Screw Cap Centrifuge Tubes are poised to revolutionize your laboratory practices. With a range of sizes, secure screw cap design, convenient bulk packaging, sterile assurance, double rewards points, and the Labware Essentials Subscription Service (LESS), we're committed to elevating your research journey.

To learn more, place an order, or sign up for LESS click HERE! Prepare to embark on a new era of efficiency, convenience, and savings in your lab.

Elevate your research. Evolve your results.

Posted in New Products

A guide to microbead milling.

By Benchmark Scientific
Aug 2, 2017

What is microbead milling? Microbead milling is a homogenization process used to break up (lyse) samples in order to release the DNA, RNA and proteins contained within the cells. Samples are placed in tubes with the appropriate grinding beads and subjected to high energy mixing. The beads impact the sample, eventually breaking it down on the cellular level releasing subcellular contents. The samples are then typically centrifuged and the lysate recovered from above the beads.

The Benchmark BeadBug™ 6 and BeadBlaster™ 24 are bead beating homogenizers, designed to rapidly homogenize small samples in disposable tubes with the aid of grinding beads. Both instruments work by physically moving tubes, samples, and grinding beads in an oscillating motion several thousand times per minute. Through this motion, the beads tear into and crush samples on impact. They are different in capacity - if only a few samples require processing a day, then the BeadBug will be sufficient for most researchers. For those with higher capacity demands or a larger lab group, the BeadBlaster 24 is more practical.

Microbead homogenizing can be effective at disrupting many different sample types. The key aspect of successfully homogenizing samples is to match the correct bead to the sample and then not to overload the disruption tube. For bead beating to be effective, the beads need to move and crash into the sample. Tubes packed with solid tissues and/or filled with buffer prevent the beads from moving freely.

General guidelines that can help to ensure that samples are homogenized effectively include:

•Do not over fill tubes. The total volume of the sample, beads, and buffer should never exceed half the volume of the tube. Less is better.

•Sample and buffer should be twice the volume of the beads.

•Detergents should be kept to a minimum as the foaming will impede the movement of the beads.

•Solid tissues should never be more than 1/20th the volume of the disruption tube, i.e., no more than 100 mg tissue in a 2 ml tube.

•If sample warming is an issue, process the sample in short bursts.

A variety of prefilled tubes are available for homogenizing samples including those with glass and Triple-Pure™ zirconium beads. Both bead types are acid washed and processed to remove nucleases and proteases. The Triple-Pure bead tubes undergo special handling during filling and are quality tested to ensure the elimination of DNase, RNase, protease, and nucleic acids. When samples are used for molecular biology applications, the high purity of the Triple-Pure beads is preferred.

Both glass (silica) and zirconium beads are effective at disrupting cells and tissues. The added density of zirconium helps to yield better homogenized samples. Benchmark has nine varieties of prefilled tubes, each which can be used with a general category of sample. These tubes are:

Standard Glass Beads, 0.1 mm – Effective for the homogenization of bacteria. Best used for cultured microbes where low cell densities are not an issue.

Standard Glass Beads, 0.5 mm – For larger microbial cells, like yeast. Effective for homogenizing yeast cultures, especially where heat labile proteins might be involved.

Standard Glass Beads 1.0 mm – For small pieces of soft tissue, fungal mycelium, and larger algal cells.

Triple-Pure Zirconium Beads, 0.1 mm – For all bacteria and low density cultures.

Triple-Pure Zirconium Beads, 0.5 mm –Effective for the homogenization of yeast and smaller algae cells. Effective for low density cultures and samples where contamination is an issue.

Triple-Pure Zirconium Beads, 1.0 mm – Use for fungal mycelium, soft tissues, very small tissue samples.

Triple-Pure Zirconium Beads, 1.5 mm – Effective for homogenizing softer animal tissues (adipose, mouse intestine, hypothalamus, and liver) as well as leaf and soft vegetables (e.g., potato).

Triple-Pure Zirconium Beads, 3.0 mm – Provides greater energy for shearing and rupturing tough samples such as muscle, lung, and kidney as well as small plant seeds, stems, and roots.

Stainless Steel Beads, 2.8 mm – Stainless steel has greater density than both glass and zirconium and can be used on very tough samples such as cornea and connective tissue and in situations where the other tube types are not effective. Metals beads are effective at homogenizing tough samples, but they also generate significant heat. Care should be taken when working with heat labile molecules. Plant extracts will often oxide rapidly in the presence of stainless steel.

Sample Types:

Bacteria – As bacteria tend to be very small, 1-3 µm in length or diameter, smaller beads normally work better for cell disruption. A culture of bacteria contains upwards of 109 cells/ml thus smaller beads (e.g., 0.1 mm) allow for greater surface area for impacting cells. This is a very significant factor in cracking bacteria. Generally the mass of the beads greatly exceeds the mass of the bacteria, thus both glass and zirconium work well. However, glass beads tend to clump more than denser zirconium beads which settle more effectively in lysates after processing. Other organisms of comparable size, such as cyanobacteria, can also be processed in a similar manner.

Yeast – The next larger microorganism after bacteria is yeast, being roughly 10+ microns in diameter or length. Beads with a diameter of 0.5 mm tend to work very well on yeast cells. Many single cell algae can be processed as if they are yeast.

Filamentous Fungi – Fungi that are not solid bodies, such as pycnidia and similar fruiting bodies, are effectively disrupted with the next size of beads. Beads of 1.0 mm are very effective for shearing mycelium.

Plant Leaves – Leaves can be homogenized readily using 1.5 and 3.0 mm zirconium beads and 2.8 mm stainless steel beads. Leaves are often harvested by cutting out the sample with a paper hole punch. A sample of this size is normally 10-20 mg in mass, thus multiple punches can be processed in one tube.

Shoots and Stalks – More resilient plant material can be effectively homogenized using the larger bead sizes. Stalks of bundled grasses and young roots and shoots can be effectively homogenized using these beads, however plants which are more mature may have significant pulp following homogenization if their lignocelluloses content is high. Sample size should not exceed 50-70 mg to ensure good disruption.

Seeds – Small fresh seeds can be processed effectively using 3.0 mm zirconium and 2.8 mm stainless steel beads. Larger seeds must first be dissected so that the sample mass is no more the 50 mg.

Soft Animal Tissue – Animal tissues can be grouped into soft and hard tissue types. The softer tissues, such as liver, adipose, and hypothalamus, can readily be disrupted using 1.5 mm beads or larger. Though easily homogenized, it is still important not to overload tubes with soft animal tissues.

Resilient Animal Tissues – Fibrous animal tissues such as muscle, lung, kidney and heart can be difficult to homogenize. By using larger beads, these tissues can be effectively disrupted to liberate RNA, DNA and proteins with very high yields. Triple-Pure 3.0 mm zirconium beads and stainless steel beads (2.8 mm) are effective for disrupting these tough tissues. Sample size is best kept under 50 mg while buffer should be no more than 200-300 µl. Many RNA extraction protocols use larger volumes of buffer, and for these processes the sample can be homogenized in less extraction buffer and then brought up to volume following homogenization.

Posted in Technical