Accuris™ qMAX™ 1st Strand cDNA Synthesis Flex Kit, 20µl volume, 50 Reactions
Availability:
In stock
SKU
PR2110-50
Description
Accuris" qMAX" 1st Strand cDNA Synthesis Flex Kit
$91.75
Accuris™ qMAX™ 1st Strand cDNA Synthesis Flex Kit, 20µl volume, 50 Reactions is available to buy in increments of 1
High quality, qPCR ready DNA is quickly generated with the Accuris qMax cDNA Synthesis Kits. Both kits
include the exceptionally thermostable qMax Reverse Transcriptase, which allows for transcription to take
place at higher temperatures (alleviating secondary structure problems) and generates a very high cDNA
yield from a very small amount of RNA. A potent RNase inhibitor maintains the integrity of the total RNA.
The perfect environment for highly efficient cDNA synthesis is provided by the supplied 5X buffer. The
buffer is optimized for superior data accuracy and reproducibility with limited starting material and lowcopy templates from a wide range of RNA sources.
include the exceptionally thermostable qMax Reverse Transcriptase, which allows for transcription to take
place at higher temperatures (alleviating secondary structure problems) and generates a very high cDNA
yield from a very small amount of RNA. A potent RNase inhibitor maintains the integrity of the total RNA.
The perfect environment for highly efficient cDNA synthesis is provided by the supplied 5X buffer. The
buffer is optimized for superior data accuracy and reproducibility with limited starting material and lowcopy templates from a wide range of RNA sources.
Our 1st Strand cDNA Synthesis Flex Kit includes four components: a high capacity Reverse Transcriptase, an optimized 5X buffer, and separate solutions of oligo (dT) primers, and random hexamer primers. This multi-component format is ideal for 10pg to 2.0µg of input RNA and allows for greater flexibility in assay design.
Features:
- Includes an MMLV derived reverse transcriptase modified for greater sensitivity and thermostability
- Optimized buffer system for superior accuracy and reproducibility
- Potent RNase inhibitor maintains sample integrity
- Choose original or flex option
Technical Specifications:
| Model Number | PR2110-50 | |
| Reactions | 50 | |
| RNA Template | 10pg to 2.0µg Total RNA | 5pg to 0.5µg mRNA |
| Random Hexaprimer or oligo (dT) | 2 µl | Use 3µl for >2µg RNA |
| PCR-grade water | to 12µl final volume | |
| 5X RT Buffer | 4µl | |
| High Capacity RT | 1.0µl | Use 2µl for >2µg RNA |
| PCR grade water | to 8µl final volume | |
| Technical Specifications |
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