Accuris SmartCheck DNA Ladders are produced from proprietary plasmids, digested to completion and purified in a multi-step chromatography process. Ladders are supplied in a ready-to-use format that includes loading buffer and tracking dyes. -Three sizes: 50bp, 100bp and 1Kb -Clear, distinct bands -Reference bands with increased intensity for easy size determination -Convenient, simply load 5µl per lane The ladders are supplied at a concentration of 0.1mg/ml, in a buffer consisting of 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol.
This product is shipped at ambient temperature. Upon receipt, immediately store at -20ºC. Ladders can be stored for up to 15 months at -20ºC or at 4ºC for up to 6 months.
The ladders are purified by a multistep chromatography method. Band sharpness is verified by agarose electrophoresis and DNA concentration is verified spectrophotometrically.
SmartCheck DNA ladders are supplied ready-touse. Briefly vortex the tube and add 5µl to the desired well in the gel. If the ladders are frozen, allow time to thaw and mix thoroughly.
The SmartCheck Ladders can be used to approximate the mass of DNA in samples of similar size by comparing the intensity of the sample band to that of the ladder. The masses of each DNA band per 5µl load are given on the back side of this insert.
Limitations of Use
For research purposes only. Not intended for therapeutic or diagnostic use. Accuris is not responsible for consequential or incidental damages, whether direct or indirect, resulting from use of this product.
- Ready-to-use formulation includes loading buffer and tracking dye
- 500µl suitable for 100 lanes (5µl per lane)
- Higher intensity reference bands
- Ultra pure production allows economical ambient shipping