Accuris™ 1 Hour Mammalian Genotyping Kit, 400 Reactions
Availability: In stock
SKU
PR1300-MG-400
Description
Accuris" 1 Hour Mammalian Genotyping Kit
$525.56
Accuris™ 1 Hour Mammalian Genotyping Kit, 400 Reactions is available to buy in increments of 1
The Accuris 1 Hour Mammalian Genotyping Kit is quick and easy add sample to the proprietary buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes.
Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.
The Accuris Mammalian Genotyping Kit is quick and easy to use add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR Accuris Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed just add sample and primers.
Features:
- DNA extraction and amplification in 1 hour.
- Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues.
- Single tube no organic solvents or clean up procedures.
- Enhanced Accuris Hot Start Polymerase included.
Technical Specifications:
| Model Number | PR1300-MG-400 | ||
| Reactions | 400 | ||
| Component | 25 µl reaction | 50 µl reaction | Final reaction |
| Accuris qMAX 2X Green Master Mix | 12.5 µl | 25 µl | 1x |
| Forward Primer (10µM) | 1 µl | 2 µl | 400 nM |
| Reverse Primer (10µM) | 1 µl | 2 µl | 400 nM |
| Supernatent from extraction step | 0.5 - 1.0µl | 1 - 2µl | Variable |
| PCR-grade water | Up to final reaction volume | ||
| Step | Temperature | Time | |
| Enzyme activation | 95°C | 1-2 minutes | |
| 35-40 cycles | 95°C 72°C | 15 seconds 15 seconds 15 seconds per Kb | |
| Technical Specifications |
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