Molecular Biology Kit, EZ-10 Spin Column Endotoxin-Free Plasmid DNA Miniprep Kit, 50 Preps

Storage

Transportation at ambient temperature. Store kits components at 4^oC. The kit is stable for one year.

Introduction

The kit provides a simple, rapid and efficient method for plasmid DNA isolation from bacteria using a rapid spin-column. DNA in lysate is selectively absorbed on the column while other impurities such as proteins and salts are eliminated. Average yield of DNA is around 10 μg. The kit also produces Endotoxin-Free plasmid by combining alkali lysis methodology with a unique Endotoxin Eliminator reagent. Final DNA is ready for further research application such as transfection, expression in animal cell strains, or as nucleotide vaccine.

Feature

• Fast.
• High purity with OD260/OD280 ratio of 1.8-2.0
• Versatile and suitable for low and high copy plasmid
• Competitive Price ü Minimum Endotoxin contaminations.

Notice:

• Before use, add the RNase A Solution to the bottle containing Solution I and mix well. Solution I with RNase should be stored at 4ºC for frequent use and at -20ºC for infrequent use.
• Solution II may form a precipitate upon storage. If necessary, dissolve the precipitate by warming the solution at 37ºC.
• Before use, add 48ml of 96-100% of ethanol to 12ml Wash Solution.
• If Liquid Endotoxin Eliminator becomes layered or turbid, please keep on ice for 10min and then mixed thoroughly