Molecular Biology Kit, EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit, 4 Preps
Availability: In stock
SKU
SK1249
Description
Molecular Biology Kits
$126.85
Molecular Biology Kit, EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit, 4 Preps is available to buy in increments of 1
Introduction
Bio Basic Plasmid DNA Extraction Maxiprep Kit is an excellent tool offering a rapid and economic method to purify plasmid DNA from bacterial cultures. This technology is based on alkaline lysis and purification by Anion-exchange chromatography. Compared with other harmful and time-consuming procedures such as phenol / chloroform extraction and ethanol precipitation, Bio Basic Plasmid DNA Extraction Kit shortens the handling time to about 2 hours. The high quality plasmid DNA can be used directly for any downstream application.
Additional MaterialsRequired
- 50 mL centrifuge tube.
- Isopropanol
- 70% Ethanol
Kit Contents
Components | SK1249 4 Preps | SK1250 20 Preps |
MAXI 1 Resuspension Solution | 55 ml | 2 x 110 ml |
MAXI 2 Cell Lysis Solution | 55 ml | 2 x 110 ml |
MAXI 3 Neutralization Solution | 55 ml | 2 x 110 ml |
MAXI 4 Equilibration Solution | 54 ml | 2 x 135 ml |
MAXI 5 Washing Solution | 4 x 55 ml | 4 x 275 ml |
MAXI 7 Elution Solution | 54 ml | 2 x 135 ml |
Liquid Endotoxin Eliminator | 8 ml | 40 ml |
RNase A (50 mg/mL) | 88 µL | 2 x 220 µL |
MAXI Column | 4 pcs | 20 pcs |
User Manual | 1 | 1 |
Important Notes
- Solutions provided in this kit contain irritants, wear gloves and lab coat when handling.
- Briefly spin RNase A tube to remove drops from the inside of the lid. Transfer tube contents into MAXI 1 Resuspension Solution bottle. Add 250 µL of MAXI 1 Resuspension Solution into RNase A tube, rinse tube inside and transfer back into MAXI 1 Resuspension Solution bottle. Store at 4°C.
- Check MAXI 2 Cell Lysis Solution before use. Warm MAXI 2 Cell Lysis Solution at 37°C if any precipitation formed. Prevent vigorous shaking of the MAXI 2 Cell Lysis Solution.
- To avoid acidification of MAXI 2 Cell Lysis Solution from CO2 in the air, close the bottle immediately after use.
Technical Specifications:
Part# | SK1249 |
Decription | EZ-500 Spin Column Endotoxin-Free Plasmid DNA Maxi-Preps Kit |
Sterile | No |
Qty | 1 |
Shipping Conditions | RT |
Qty | 4 Preps |
Technical Specifications |
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