GelNest Matrix, with Phenol Red, Growth Factor Reduced, LDEV-Free, 5 mL/Bottle, 1 Bottle/Bag
GelNestâ„¢ Matrix is prepared from basement membrane components extracted from mousetumor tissues. The main components include laminin, type IV collagen , heparan sulfate proteoglycan, etc. These components can provide the support and signals required for cell adhesion, differentiation, and proliferation. They can also simulate the characteristics of the basement membrane in a physiological environment and improve the success rate and effect of cell culture.
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In addition to basement membrane components, GelNestâ„¢ Matrix is also rich in a variety of growth factors. These growth factors can promote cell differentiation, proliferation, and migration, further mimicking cell signaling pathways and interactions in physiological environments. GelNestâ„¢ Matrix has a wide range of application prospects, especially in tissue engineering, cell culture and research. It can be used for research on organoid culture, stem cell differentiation, angiogenesis, migration or invasion, and in vivo tumorigenesis.
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Product parameters:
| Source | Mouse tumor tissue basement membrane components |
| Formulation* | Growth factor reduced. 211232 with phenol red, 211242 without phenol red. |
| Protein Concentration | See label, or please download the COA from our official website to obtain a lot-specific concentration. |
| Appearance | GelNest™ Matrix is liquid at 4°C but forms a gel at 37°C. Phenol red-containing gel appears bright yellow when frozen, and red at temperatures above 0ºC. |
| Applications | Suitable for 2D primary cell culture, organoid construction, culture, differentiation, invasion assays, stem cell culture, in vitro and in vivo angiogenesis experiments. |
| Storage and Shelf Life | Store in a refrigerator at -20℃ (frost-free function off) or a -80℃ freezer for up to 2 years. It is recommended to aliquot the thawedproduct into single-use portions and store it in -20°C or -80°C for up to 2 years. |
| Precautions | GelNest™ Matrix will start to solidify when the temperature is higher than 10°C. Please try to operate on ice as much as possible, and it is recommended to pre-cool the consumables that directly contact the gel, such as pipette tips. |
*Please use phenol red-free matrix gel for colorimetric analysis. For the preparation of a more defined basement
membrane coating, it is recommended to use growth factor reduced matrix gel.
Experimental procedures:
| Application Type | Dilution Factor | Final Concentration | Volume Added | Experiments |
| Thin Gel | 1:10 | >1mg/mL | 50μL/cm2 | 2D primary cell culture |
| 1:50 | >0.1mg/mL | Invasion assay with cell culture inserts |
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| 1:100 | >0.01mg/mL | 300μL/cm2 | Stem cell culture | |
| Thick Gel | Gel : Cell Mixture<7:3 | >7mg/mL | 150 - 200μL/cm2 | Organoid culture |
| No Dilution | >10mg/mL | Angiogenesis |
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Technical Specifications
| Model Number | 211232 |
| Growth Factor | Low |
| Product | GelNestâ„¢ Matrix, low growth factor |
| Phenol Red | Yes |
| Recmmended Applications | 2D, 3D cell culture with higher accuracy requirements for matrix components and requires |
| Specifications | 5mL/bottle, 1 bottle/box |
| Corning Cross Reference #Â | 356230 |
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Storage and Operation Instructions
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To store GelNest™ Matrix, it is recommended to keep it in a -20°C freezer until ready for use. Prior to initial use, thaw the gel and divide it into single-use portions. Store these portions in a -80°C freezer for a maximum of 2 years. Please note that GelNest™ Matrix Gel should not be stored in a frost-free freezer.
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GelNest™ Matrix is in a liquid state at 4°C and transitions into a gel state at 37°C. It starts to solidify into a gel when the temperature exceeds 10°C. For optimal performance, it is advised to pre-chill the pipette tips and conduct the procedures on ice.
| Technical Specifications |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Growth Factor | Low | ||||||||||||||
| Phenol Red | Yes | ||||||||||||||
| Corning item # | 356230 | ||||||||||||||
| Recommended Applications | 2D, 3D cell culture with higher accuracy requirements for matrix components and requires colorimetric identification or sensitivity to steroids |
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