GelNest Matrix High for Organ-like Structures, with Phenol Red-Free, LDEV-Free, 5 mL/Bottle, 1 Bottle/Bag
GelNestâ„¢ Matrix is prepared from basement membrane components extracted from mousetumor tissues. The main components include laminin, type IV collagen , heparan sulfate proteoglycan, etc. These components can provide the support and signals required for cell adhesion, differentiation, and proliferation. They can also simulate the characteristics of the basement membrane in a physiological environment and improve the success rate and effect of cell culture.
In addition to basement membrane components, GelNestâ„¢ Matrix is also rich in a variety of growth factors. These growth factors can promote cell differentiation, proliferation, and migration, further mimicking cell signaling pathways and interactions in physiological environments. GelNestâ„¢ Matrix has a wide range of application prospects, especially in tissue engineering, cell culture and research. It can be used for research on organoid culture, stem cell differentiation, angiogenesis, migration or invasion, and in vivo tumorigenesis.
Product parameters:
| Source | Mouse tumor tissue basement membrane components |
| Formulation* | Without phenol red. Optimized for organoid culture. |
| Protein Concentration | See label, or please download the COA from our official website to obtain a lot-specific concentration. |
| Appearance | GelNest™ Matrix is liquid at 4°C but forms a gel at 37°C. |
| Applications | Validated with organoid culture experiment. Suitable for organoid development, cultivation and differentiation. |
| Storage and Shelf Life | Store in a refrigerator at -20℃ (frost-free function off) or a -80℃ freezer for up to 2 years. It is recommended to aliquot the thawedproduct into single-use portions and store it in -20°C or -80°C for up to 2 years. |
| Precautions | GelNest™ Matrix will start to solidify when the temperature is higher than 10°C. Please try to operate on ice as much as possible, and it is recommended to pre-cool the consumables that directly contact the gel, such as pipette tips. |
Experimental procedures:
Please determine the specific experimental steps based on cell types, culture conditions, and application experience.
Technical Specifications
| Model Number | 211282 |
| Growth Factor | Low |
| Product | GelNestâ„¢ Matrix, for organ-like structures, without Phenol Red |
| Phenol Red | No |
| Recmmended Applications | Organoid culture and differentiation |
| Specifications | 5mL/bottle, 1 bottle/box |
| Corning Cross Reference # | 356255(10mL only) |
Storage and Operation Instructions
To store GelNest™ Matrix, it is recommended to keep it in a -20°C freezer until ready for use. Prior to initial use, thaw the gel and divide it into single-use portions. Store these portions in a -80°C freezer for a maximum of 2 years. Please note that GelNest™ Matrix Gel should not be stored in a frost-free freezer.
GelNest™ Matrix is in a liquid state at 4°C and transitions into a gel state at 37°C. It starts to solidify into a gel when the temperature exceeds 10°C. For optimal performance, it is advised to pre-chill the pipette tips and conduct the procedures on ice.
| Technical Specifications |
| ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Growth Factor | Low | ||||||||||||||
| Phenol Red | No | ||||||||||||||
| Corning item # | 354277 | ||||||||||||||
| Recommended Applications | hESC stem cell culture |
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