Accuris qMax Green with Blue Tracking Dye, Low Rox qPCR Mix, 500 x 20µl reactions

Accuris qMax Green with Blue Tracking Dye, Low Rox qPCR Mix, 500 x 20µl reactions

Accuris qMax Green with Blue Tracking Dye, Low Rox qPCR Mix, 100 x 20µl reactions

Accuris qMax Green with Blue Tracking Dye, Low Rox qPCR Mix, 100 x 20µl reactions

Accuris qMax Green  with Blue Tracking Dye, Low Rox qPCR Mix, 1000 x 20µl reactions

Accuris qMax Green with Blue Tracking Dye, Low Rox qPCR Mix, 1000 x 20µl reactions

Accuris qMax Green with Blue Tracking Dye, Low Rox qPCR Mix, 500 x 20µl reactions

Availability: In stock
SKU
PR2000-L-500
$297.85
Accuris qMax Green with Blue Tracking Dye, Low Rox qPCR Mix, 500 x 20µl reactions is available to buy in increments of 1

Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fact cycling and excellent reproducibility. Accuris Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers and pH that results in earlier Ct values and a high specificity across a broad dynamic range.  An inert blue dye is also mixed into the qMAX Green to allow easy visualization during pipetting.

 

 

Features:

  • Superior sensitivity and fast cycling with exceptional results.
  • Ideal for low copy number templates.
  • Early Ct values and detection across a broad dynamic range.
  • Ready to use 2x mastermix.
  • Includes Accuris Hot Start Taq Polymerase for greater specificity and accuracy.
  • Inert, blue tracking dye assists with accurate pipetting.

 

 

 

Technical Specifications:

Model Number PR2000-L-500
Rox Low
Reactions qty 500
Component 20 µl reaction Final concentration
Accuris qMAX 2X Green Master Mix 10 µl 1x
Forward Primer (10µM) 0.8 µl 400 nM
Reverse Primer (10µM) 0.8 µl 400 nM
Template DNA <100 ng cDNA, <1 µg genomic variable
PCR-grade water Up to 20µl final reaction volume
Step Temperature Time
Initial denaturation 95°C 2-5min. (3+ min. for gemonic DNA)
40 cycles* 95°C
60° - 65°C
5 seconds
10-40 seconds
Melt Anaylsis (optional)

 *Do not use temperatures below 60º or exceed 30 seconds

 

More Information
Technical Specifications
Model Number PR2000-L-500
Rox Low
Reactions qty 500
Component 20 µl reaction Final concentration
Accuris qMAX 2X Green Master Mix 10 µl 1x
Forward Primer (10µM) 0.8 µl 400 nM
Reverse Primer (10µM) 0.8 µl 400 nM
Template DNA <100 ng cDNA, <1 µg genomic variable
PCR-grade water Up to 20µl final reaction volume
Step Temperature Time
Initial denaturation 95°C 2-5min. (3+ min. for gemonic DNA)
40 cycles* 95°C
60° - 65°C
5 seconds
10-40 seconds
Melt Anaylsis (optional)

 *Do not use temperatures below 60º or exceed 30 seconds