5-Layer Cell Culture Flask, Straight Neck, Plug Seal Cap, TC Treated, 1/pk, 8/cs

5-Layer Cell Culture Flask, Straight Neck, Plug Seal Cap, TC Treated, 1/pk, 8/cs

Sterileware Double-Ended Pestle, 4

Sterileware Double-Ended Pestle, 4", Plastic

5-Layer Cell Culture Flask, Straight Neck, Plug Vent Cap, TC Treated, 1/pk, 8/cs

5-Layer Cell Culture Flask, Straight Neck, Plug Vent Cap, TC Treated, 1/pk, 8/cs

5-Layer Cell Culture Flask, Straight Neck, Plug Seal Cap, TC Treated, 1/pk, 8/cs

Availability: In stock
SKU
731001
Description

Multi-Layer Culture Flask

$202.22
5-Layer Cell Culture Flask, Straight Neck, Plug Seal Cap, TC Treated, 1/pk, 8/cs is available to buy in increments of 1

Multi-layer flasks are manufactured under TUV ISO 9001:2008 certification guidelines in a U.S. FDA registered facility. All sterile products are manufactured in a 100,000 grade super clean production facility and are certified sterile, DNase and RNase Free, and Non-pyrogenic. Our cell culture flasks are an ideal solution for enhanced cell attachment and growth of a variety of primary cells and transformed cells in serum free or serum-containing cultures.

Features:

  • High clarity medical grade polystyrene material
  • Sterilized by E-Beam SAL=10-6
  • Nonpyrogenic
  • Packaged in sterile bag
  • Two types of cap style : vent caps & plug seal cap
  • Vent caps with 0.22um hydrophobic filters for gas exchange without contamination
  • TC treated , good for cell attachment and growth.

ADDING MEDIA AND PREPARING CELL SUSPENSION

1. Mix cell suspension with medium: Prepare cell suspension of required concentration in a container and mix. Recommended volume is about 30- 50 mL per layer.

2. Add the mixed liquid into the Multi-layer Flask slowly with serological pipettes. To avoid foams and bubbles, allow liquid stream to flow along the slope of the Multi-layer Flask. ( Save a little liquid in pipette for each dosing .)
TipsA 10 mL pipette allows media to be dispensed at the bottom of the vessel. A 25 mL pipette allows media to be dispensed just past the NEST Logo.

3. Hold the Multi-layer Flask upright with the Logo facing you and tilt clockwise to a 45° angle on a flat work surface to partition the liquid into each layer.

4. While holding the Multi-layer Flask at a 45° angle, gently lay it flat onto the work surface with logo facing up.

5. After placing the Multi-layer Flask flat on a work surface, gently rock back and forth and side-to-side to distribute cells evenly onto culture surfaces.
Tips: Take care to avoid foaming of medium, and not to spill liquid from each layer.

6. Repeat Step 3 to put the flask quickly and slightly into the incubator . Then, lay it flat as shown in Step 4 .

Media Removal

You may choose to either aspirate or pour the media from Multi-layer Flask.

7. Aspirating method: To aspirate or remove media, tilt Multi-layer Flask, with the NEST Logo facing you, counter-clock wise to a 45° angle while inverting the Multi-Flask toward you. Then, tilt Multi-layer Flask to the right, continuing to aspirate all residual media.

8. Pouring method: With Logo facing you, pour spent media from Multi-layer Flask
Tips: Aspirate media using a NEST 2mL or 10mL aspirating pipette.

Cell Harvesting

9. Wash with buffer for one time and add dissociating reagent (e5 mL per layer). Then, follow Steps 3-4 to distribute to dissociating reagent to each layer.
10. Neutralize with inactivating solution and mix following Steps 3-4. Gently swirl to dislodge cells completely.
11. Follow Step 7 Aspirating Method protocol and collect cell suspension using a NEST 10mL serological pipet .
12. Follow Step 8 Pouring Method. Pour de¬tached cell suspension into a NEST conical tube. 13. Rinse with additional wash buffer as needed.

Technical Specifications:

Model Number731001
Cell Growth Area 870 cm2
Cap StylePlug Seal Cap
Temperature RangeApprox.-86 to+64
Shelf Life3 Years after date of production ( ensure package is in good )
SterileYes
/Pack1
/Case8
More Information
Technical Specifications
Model Number731001
Cell Growth Area 870 cm2
Cap StylePlug Seal Cap
Temperature RangeApprox.-86 to+64
Shelf Life3 Years after date of production ( ensure package is in good )
SterileYes
/Pack1
/Case8