5-Layer Cell Culture Flask, Straight Neck, Plug Seal Cap, TC Treated, 1/pk, 8/cs
Multi-Layer Culture Flask
Multi-layer flasks are manufactured under TUV ISO 9001:2008 certification guidelines in a U.S. FDA registered facility. All sterile products are manufactured in a 100,000 grade super clean production facility and are certified sterile, DNase and RNase Free, and Non-pyrogenic. Our cell culture flasks are an ideal solution for enhanced cell attachment and growth of a variety of primary cells and transformed cells in serum free or serum-containing cultures.
Features:
- High clarity medical grade polystyrene material
- Sterilized by E-Beam SAL=10-6
- Nonpyrogenic
- Packaged in sterile bag
- Two types of cap style : vent caps & plug seal cap
- Vent caps with 0.22um hydrophobic filters for gas exchange without contamination
- TC treated , good for cell attachment and growth.
ADDING MEDIA AND PREPARING CELL SUSPENSION
1. Mix cell suspension with medium: Prepare cell suspension of required concentration in a container and mix. Recommended volume is about 30- 50 mL per layer.
2. Add the mixed liquid into the Multi-layer Flask slowly with serological pipettes. To avoid foams and bubbles, allow liquid stream to flow along the slope of the Multi-layer Flask. ( Save a little liquid in pipette for each dosing .)
TipsA 10 mL pipette allows media to be dispensed at the bottom of the vessel. A 25 mL pipette allows media to be dispensed just past the NEST Logo.
3. Hold the Multi-layer Flask upright with the Logo facing you and tilt clockwise to a 45° angle on a flat work surface to partition the liquid into each layer.
4. While holding the Multi-layer Flask at a 45° angle, gently lay it flat onto the work surface with logo facing up.
5. After placing the Multi-layer Flask flat on a work surface, gently rock back and forth and side-to-side to distribute cells evenly onto culture surfaces.
Tips: Take care to avoid foaming of medium, and not to spill liquid from each layer.
6. Repeat Step 3 to put the flask quickly and slightly into the incubator . Then, lay it flat as shown in Step 4 .
Media Removal
You may choose to either aspirate or pour the media from Multi-layer Flask.
7. Aspirating method: To aspirate or remove media, tilt Multi-layer Flask, with the NEST Logo facing you, counter-clock wise to a 45° angle while inverting the Multi-Flask toward you. Then, tilt Multi-layer Flask to the right, continuing to aspirate all residual media.
8. Pouring method: With Logo facing you, pour spent media from Multi-layer Flask
Tips: Aspirate media using a NEST 2mL or 10mL aspirating pipette.
Cell Harvesting
9. Wash with buffer for one time and add dissociating reagent (e5 mL per layer). Then, follow Steps 3-4 to distribute to dissociating reagent to each layer.
10. Neutralize with inactivating solution and mix following Steps 3-4. Gently swirl to dislodge cells completely.
11. Follow Step 7 Aspirating Method protocol and collect cell suspension using a NEST 10mL serological pipet .
12. Follow Step 8 Pouring Method. Pour de¬tached cell suspension into a NEST conical tube. 13. Rinse with additional wash buffer as needed.
Technical Specifications:
Model Number | 731001 |
Cell Growth Area | 870 cm2 |
Cap Style | Plug Seal Cap |
Temperature Range | Approx.-86 to+64 |
Shelf Life | 3 Years after date of production ( ensure package is in good ) |
Sterile | Yes |
/Pack | 1 |
/Case | 8 |
Technical Specifications |
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