Accuris qMAX Probe One-Step RT-qPCR Kit, Low Rox, 100 reactions
One-Step RT-PCR Kit
Gene Expression Analysis & Pathogen Detection
The qMAX" Probe One-Step RT-qPCR Kit allows for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates.
The optimized buffer includes a powerful RNase inhibitor and an extremely thermostable MMLV-derived reverse transcriptase for robust first strand cDNA synthesis. Accuris Hot Start Taq Polymerase employs rapid antibody-mediated hot-start activation, which allows for reaction preparation at room temperature.
The Kit can be used to quantify a specific target RNA from either total RNA or mRna whiled reducing the number of pipetting steps due to the one tube formation.
The qMAX" Probe One-Step RT-qPCR Kit requires little, if any, optimization. Formulated for probe-detection technology, including TaqMan®, Scorpions® and molecular beacon probes, it delivers early quantification cycle values (Ct) and broad range detection for high sensitivity, speed, reliability and reproducibility. Coupled with a processive enzyme chemistry, the One-Step kit provides unmatched performance in multiplexing.
Features:
- RNA to qPCR in one tube in one step
- Enhanced performance and sensitivity
- Simplified workflow and fast results
- Unmatched multiplexing performance
- Compatible with all real time cyclers
Technical Specifications:
Model Number | PR2121-L-100 | |
Rox | Low | |
Reactions | 100 | |
Component | 20 ¼l reaction | Final concentration/Notes |
2x qMax One-Step Mix | 10 µl | 1x |
Forward Primer (10¼M) | 0.8 ¼l | 400 nM |
Reverse Primer (10¼M) | 0.8 ¼l | 400 nM |
Probe (10µM) | 1.0 - 2.0 µL | 1x or 2x |
20x RTase Blend | 1.0 ¼l | 1x Add prior to RNA |
Template RNA | 10 pg to 100 ng Total RNA | >0.01 pg mRNA |
PCR grade water | to final reaction volume |
Technical Specifications |
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