Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 100 Reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 100 Reactions

Accuris One-Step RT-PCR Kit, 100 Reactions

Accuris One-Step RT-PCR Kit, 100 Reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 1000 Reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 1000 Reactions

Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 100 Reactions

Availability: In stock
SKU
PR2120-L-100
Description

One-Step RT-PCR Kit

$153.56
Accuris qMAX Green One-Step RT-qPCR Kit, Low Rox, 100 Reactions is available to buy in increments of 1

Accurate & Fast Gene Expression Analysis

The Accuris qMAX" Green One-Step RT-qPCR Kit allows for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified by the one tube formulation of ready-to-use 2X qPCR master mix and reverse transcriptase.

The optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLVderived reverse transcriptase for robust first strand cDNA synthesis. Accuris Hot Start Taq Polymerase is the engine for qPCR, while qMAX Green allows for dye based detection. qMAX Green" exhibits greater fluorescence upon binding to double stranded DNA than other green dyes while exhibiting minimal PCR inhibition. An inert blue dye is included in the mix to help simplify pipetting and reduce errors.

The Accuris qMAX Green One-Step qPCR Kit is compatible with standard and fast cycling protocols and provides increased sensitivity, speed and reproducibility for a broad range of samples and targets. The polymerase mix is available with low or high level of ROX reference dye for compatability with all qPCR instruments.

Features:

  • RNA to qPCR in one tube, one step
  • Enhanced performance and sensitivity
  • Simplified workflow and fast results
  • Advanced buffer formulation with powerful RNase inhibitors
  • Compatible with all real time cyclers

amplification

Technical Specifications:

Model NumberPR2120-L-100
RoxLow
Reactions100
Component20 ¼l reactionFinal concentration/Notes
2x qMax One-Step Mix10 µl1x
Forward Primer (10¼M)0.8 ¼l400 nM
Reverse Primer (10¼M)0.8 ¼l400 nM
Probe (10¼M)1.0 - 2.0 ¼L1x or 2x
20x RTase Blend1.0 ¼l1x Add prior to RNA
Template RNA10 pg to 100 ng Total RNA>0.01 pg mRNA
PCR grade waterto final reaction volume

More Information
Technical Specifications
Model NumberPR2120-L-100
RoxLow
Reactions100
Component20 ¼l reactionFinal concentration/Notes
2x qMax One-Step Mix10 µl1x
Forward Primer (10¼M)0.8 ¼l400 nM
Reverse Primer (10¼M)0.8 ¼l400 nM
Probe (10¼M)1.0 - 2.0 ¼L1x or 2x
20x RTase Blend1.0 ¼l1x Add prior to RNA
Template RNA10 pg to 100 ng Total RNA>0.01 pg mRNA
PCR grade waterto final reaction volume