$ Molecular Biology Kit, One Step-RNA Reagent, 25ml

Molecular Biology Kit, One Step-RNA Reagent, 25ml

Molecular Biology Kit, EZ-10 Total RNA Miniprep Kit (with Proteinase K), 50 Preps

Molecular Biology Kit, EZ-10 Total RNA Miniprep Kit (with Proteinase K), 50 Preps

Molecular Biology Kit, One Step-RNA Reagent, 100ml

Molecular Biology Kit, One Step-RNA Reagent, 100ml

Molecular Biology Kit, One Step-RNA Reagent, 25ml

Availability: In stock
SKU
BS409A
Description

Molecular Biology Kits 

$31.54
Molecular Biology Kit, One Step-RNA Reagent, 25ml is available to buy in increments of 1

Description:

ONE STEP-RNA Reagent is a ready- to- use reagent for the isolation of total RN A from cells and tissues. The reagent, a mono -phasic solution of phenol and guanidine isothiocyanate , is an improvement to the single - step RNA isolation method developed by Chomczynski and Sacchi. During sample homogenization or lysis, ONE STEP-RNA Reagent maintains the integrity of the RNA , while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation , separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase , the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase , the DN A and protein s in the sample can be recovered by sequential precipitation. Precipitation with ethanol yields DNA from the interphase, and an additional precipitation with isopropyl alcohol yields proteins from the organic phase. Co-purification of the DNA maybe useful for normalizing RNA yields from sample to sample.

This technique performs well with small quantities of tissue (50 ~100 mg) and cells (5 ×106) , and large quantities of tissue ( ³ 1 g ) and cells ( >10 7 ) , of human , animal, plant , or bacterial origin. The simplicity of the ONE STEP-RNA Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA isolated by ONE STEP-RNA Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis , dot blot hybridization , poly (A)+ selection , in vitro translation, RNase protection assay, and molecular cloning. For use in the polymerase chain reaction (PCR ), treatment of the isolated RNA with amplification grade DNase I is recommended when the two primers lie within a single exon.

O NE STEP-RNA Reagent facilitates isolation of a variety of RNA species of large or small molecular size. For example , RNA isolated from rat liver, electrophoresed on an agarose gel, and stained with ethidium bromide , shows discrete bands of high molecular weigh t RN A be t ween 7 k b and 1 5 kb in size, (composed of mRNA’s and hnRNA’s) two predominant ribosomal RNA bands at ~5 kb (28S) and a t ~ 2 k b (18 S ) , and lo w molecular weight RNA between 0.1 and 0.3 k b ( tRNA , 5S) . The isolated RN A has an A260 /280 ratio of 1.6 ~ 1.8 when diluted into distilled water. The expected yield of RNA per mg of tissue is: liver and spleen, 6~10mg; kidney, 3~4mg; skeletal muscles and brain, 1~5mg; placenta, 1~4mg. The expected yield of RNA from 1×10 6 Cult u red cells is : epithelial cells , 8 ~15 mg ; fibroblast s, 5~7 × mg.

Reagents required , but not supplied:

  • Chloroform
  • Isopropyl alcohol
  • 75% Ethanol (in DEPC - treated water)
  • RNase - free water or 0.5% SDS solution [To prepare RNase - free water , draw water int o RNase - free glass bottles. Add diethylpyrocarbonate ( DEPC ) to 0 .01% ( v /v ). Let stand over night and autoclave. The SDS solution must be prepared using DEPC-treated autoclaved water.]
 
Technical Specifications:
 
Part#BS409A
Decription

One Step-RNA Reagent

SterileNo
Storage Conditions-15 to -20C
Qty25ml
Refrigeration RequirementsFreezer
Shipping ConditionsICE

 

 

 

RNA Mini Preps Kits Handbook  

More Information
Technical Specifications
Part#BS409A
Decription

One Step-RNA Reagent

SterileNo
Storage Conditions-15 to -20C
Qty25ml
Refrigeration RequirementsFreezer
Shipping ConditionsICE