Molecular Biology Kit, EZ-10 Total RNA Miniprep Kit (with Proteinase K), 50 Preps

Introduction:

The EZ-10 Total RNA Mini-Preps Kit allows efficient purification of total RNA form various samples, total RNA is easily purified from animal or human cells and tissues using a simple spin format.

Biological samples are first lysed and homogenized in denaturing guanidine-containing buffer (Buffer RLT), which immediately inactivates DNases and RNases as well as proteases to ensure isolation of intact RNA.  RNA in the whole homogenate is selectively absorbed on EZ-10 Spin Column, all protein and other components are removed in the flow-through.  Remaining contaminants and salts are efficiently washed away.  Purified RNA is eluted in RNase-free water has OD₂₆₀/OD₂₈₀ ratios 1.9-2.1 (measured in 10mM Tris-Cl pH 7.5) and is ideal for use in most downstream applications including Northern blotting, RT-PCR, Quantitative PCR, Poly (A) RNA selection and Array analysis.

Features:

• Fast.  Using a rapid spin column format, the whole procedure takes less than 20 minutes.
• High Quality of RNA.  Purified RNA has an OD₂₆₀/OD₂₈₀ ratio of 1.9-2.0
• Versatile.  Suitable for purification of total RNA from various samples such as bacteria, fungi, animals and some of plants.
• Economic

Materials Supplied by User

• Microcentrifuge capable of at least 12,000 x g
• RNase-free pipets, pipet tips and vortexer 
• RNase-free microcentrifuge tubes (1.5 or 2 ml)
• Ethanol (96-100%)
• RNase-free water

Lysozyme Solution:

400 μg/ml Iysozyme in RNase-free water for Gram-negative bacterium

3 mg/ml Iysozyme in RNase-free water for Gram-positive bacterium

Components:

Storage:

Buffer RLT should be stored at 4^oC and the others are stored at room temperature.  The kit is stable for 1 year under these conditions.

Proteinase K solution is stable at room temperature for 6 months, store at 2-8^oC for long term.

Notes:

Before using for the first time add 4 ml ethanol to 36 ml DW solution and 48 ml ethanol to 12 ml RPE solution to make a working solution.

Salt precipitation in Buffer RLT and DW Solution (concetrate) may occur at low temperature.  Redissolve the precipitate by warming the solution at 56^oC, then cool back down to room temperature before use.

Care must be taken when working with RNA.  It is important to maintain an RNase-free environment starting with RNA sample preparation and continue through purification and analysis.  Use RNase free tubes, tips, gels.  Wear gloves at all time.

Quality Control

Each lot of EZ-10 Total Rna Mini-Preps Kit is tested against predetermined specifications to ensure consistent product quality.

Product Info