Molecular Biology Kit, EZ-10 Spin Column Viral Total RNA Extraction Kit, 50 Preps

Introduction:

The kit is simplifies isolation of viral RNA from cell-free body fluids with fast spin-column format. No phenol/chloroform extraction is required. Viral RNA binds specifically to the silica membrane while contaminants are removed in the flow-through. PCR inhibitors such as divalent cations and proteins are completely removed in two efficient wash steps, leaving pure viral RNA to be eluted in RNasefree Water. Purified RNA is ready to use in RT-PCR, Northern blotting or other downstream applications.

Features:

• Fast. Using a rapid spin column format, the entire procedure takes about 20 minutes.
• High Yield. The recovery yield of viral RNA is generally >85%
• Versatile. Suitable for purification of viral RNA from a wide range of specimens, including serum, plasma, cell culture media, and milk.
• Non-toxic: No phenol/ chloroform are used.

Components:

Universal RPE Solution is supplied in a concentrated form, before use, add 48 ml 96-100% ethanol to 12 ml concentrated universal RPE solution and mix well.

Storage:

The kit is valid for 1 year at 4°C.