Molecular Biology Kit, EZ-10 Spin Column Fungal RNA Miniprep Kit, 50 Preps

Introduction

The kit is valid for 1 year at 4°C. Introduction The kit is designed for preparation of high quality total RNA from a wide range of fungal species. Fungal samples are lysed and homogenized by Buffer Rlysis-FG. RNA in the whole homogeneity is selectively absorbed on spin column and other impurities are washed away. Total RNA is eluted from the membrane in the presence of RNase-Free Water in the final step. 3-5μg total RNA can be purified from 30mg filamentous fungi using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection and in vitro translation.

Features

• Fast. Using a rapid spin-column format, the entire procedure takes approx 20 minutes.
• High quality of RNA. OD260/280 of purified RNA is generally >1.8.
• Intact RNA: NO RNA degradation and integrity maintained.
• Economic

Materials Supplied by User:

• Microcentrifuge capable of at least 12,000 × g
• RNase-Free pipets and pipet tips
• Vortexer
• RNase-Free Ethanol (96-100%)
• RNase-Free Microcentrifuge tubes (1.5 ml or 2 ml)

*Universal GT Solution and Universal NT Solution are supplied in a concentrated form, before use; add 12 ml 96-100% ethanol to 18 ml concentrated universal GT solution and 24 ml 96-100% ethanol to 6 ml concentrated universal NT solution to make a work solution.

NOTE: Care must be taken when working with RNA. It is important to maintain an RNAse-free environment starting with RNA sample preparation and continue through purification and analysis. Use RNAse free tubes, tips, gels. Wear gloves at all times.

Product Info