Molecular Biology Kit, EZ-10 Spin Column Blood RNA Miniprep Kit, 50 Prep

Introduction

EZ-10 Column Blood RNA Purification Kit provides a simple spin column technique for preparation of high quality, high-purity intact total RNA. The reagent contains disruptive and protective properties of guanidine isothiocyanate and β-mercaptoethanol to inactivate the ribonucleases present in cell extracts. RNA in the whole homogeneity is selectively absorbed on spin column and other impurities are washed away. Total RNA is eluted from the membrane in the presence of RNase-free water. 5-15 μg total RNA can be purified from 200 μl of anticoagulated blood sample using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly (A) selection and in vitro translation.

Features:

• Fast. Using a rapid spin-column format, the entire procedure takes approx 15 minutes.
• High Purity of RNA. OD260/OD280 ratio of purified RNA is generally > 1.9.
• Compatible with downstream applications such as Northern Blots, cDNA synthesis, RT-PCR and qRT-PCR.
• High Quality RNA. Buffer Rlysis-AG maintains the integrity of the RNA, no degradation.
• Economic.

Materials Supplied by User:

• Microcentrifuge capable of at least 12,000 × g
• RNase-Free pipets and pipet tips
• Vortexer
• RNase-Free Ethanol (96-100%)
• RNase-Free Microcentrifuge tubes (1.5 ml or 2 ml)

*Universal GT Solution and Universal NT Solution are supplied in a concentrated form, before use; add 12 ml 96-100% ethanol to 18 ml concentrated universal GT solution and 24 ml 96-100% ethanol to 6 ml concentrated universal NT solution to make a work solution.

NOTE: Care must be taken when working with RNA. It is important to maintain an RNAse-free environment starting with RNA sample preparation and continue through purification and analysis. Use RNAse free tubes, tips, gels. Wear gloves at all times.

Product Info