Molecular Biology Kit, EZ-10 Spin Column Animal Total RNA Miniprep Kit, 250 Prep

Molecular Biology Kit, EZ-10 Spin Column Animal Total RNA Miniprep Kit, 250 Prep

Molecular Biology Kit, EZ-10 Spin Column Animal Total RNA Miniprep Kit, 50 Prep

Molecular Biology Kit, EZ-10 Spin Column Animal Total RNA Miniprep Kit, 50 Prep

Molecular Biology Kit, EZ-10 Spin Column Blood RNA Miniprep Kit, 50 Prep

Molecular Biology Kit, EZ-10 Spin Column Blood RNA Miniprep Kit, 50 Prep

Molecular Biology Kit, EZ-10 Spin Column Animal Total RNA Miniprep Kit, 250 Prep

Availability: In stock
SKU
BS2322
Description

Molecular Biology Kits 

$221.34
Molecular Biology Kit, EZ-10 Spin Column Animal Total RNA Miniprep Kit, 250 Prep is available to buy in increments of 1

Introduction

EZ‐10 Column Animal total RNA Purification Kit provides a simple spin column technique for preparation of high quality, high‐purity intact total RNA. The reagent contains disruptive and protective properties of guanidine isothiocyanate and β‐mercaptoethanol to inactivate the ribonucleases present in cell extracts. RNA in the whole homogeneity is selectively absorbed on spin column and other impurities are washed away. Total RNA is eluted from the membrane in the presence of RNase‐free water.

5‐15 μg total RNA can be purified from 25 mg animal issue using this kit. Purified RNA is ready for most downstream applications such as RT‐PCR, Northern Blotting, Poly (A) selection and in vitro translation.

Storage:

2 years at room temperature.

Features:

  • Fast. Using a rapid spin‐column format, the enre procedure takes approx 15 minutes.
  • High Purity of RNA. OD260/OD280 rao of purified RNA is generally > 1.9.
  • Compable with downstream applicaons such as Northern Blots, cDNA synthesis, RT‐PCR and qRT‐PCR.
  • High Quality RNA. Buffer Rlysis‐AG maintains the integrity of the RNA.
  • Economic.

Materials Supplied by User:

  • Microcentrifuge capable of at least 12,000 × g
  • RNase‐Free pipets and pipet ps
  • Vortexer
  • RNase‐Free Ethanol (96‐100%)
  • RNase‐Free Microcentrifuge tubes (1.5ml or 2ml)

 

Part Number

BS82312

BS82322

Size

50 Preps

250 Preps

Buffer Rlysis-AG

25ml

125 ml

Universal GT Solution*

18 ml

90 ml

Universal NT Solution*

6 ml

30 ml

Rnase-free Water

5 ml

25 ml

EZ-10 Spin Column

50

250

2 ml Collection Tube

50

250

Protocol

1

1

*Universal GT Solution and Universal NT Solution are supplied in a concentrated form. Before use, add 12ml or 60ml 96‐100% ethanol to 18 ml or 90ml concentrated universal GT solution and 24 ml 96‐100% ethanol to 6ml concentrated universal NT solution.

NOTE: Care must be taken when working with RNA. It is important to maintain an RNAse‐free environment starting with RNA sample preparation and continue through purification and analysis. Use RNAse free tubes, ps, gels. Wear gloves at all Microcentrifuge capable of at least 12,000 × g RNase‐Free pipets and pipet ps Vortexer RNase‐Free Ethanol (96‐100%) RNase‐Free Microcentrifuge tubes (1.5ml or 2ml)

 

Technical Specifications:
 
Part#BS82322
Decription
EZ-10 Spin Column Animal Total RNA Miniprep Kit
SterileNo
Storage Conditions18 to 25C
Qty1
Shipping ConditionsRT
Size250 Preps

 

Product Info

More Information
Technical Specifications
Part#BS82322
Decription
EZ-10 Spin Column Animal Total RNA Miniprep Kit
SterileNo
Storage Conditions18 to 25C
Qty1
Shipping ConditionsRT
Size250 Preps