Molecular Biology Kits
Introduction
EZ-10 Total RNA Mini-Preps Kit allows efficient purification of total RNA from various samples, total RNA is easily purified from animal or human cells and tissues using a simple spin format.
Biological samples are first lysed and homogenized in denaturing guanidine-containing buffer (Buffer RLT), which immediately inactivates DNases and RNases as well as proteases to ensure isolation of intact RNA. RNA in the whole homogenate is selectively absorbed on EZ-10 Spin Column, all protein and other components are removed in flow-through. Remaining contaminants amd salts are efficiently washed away. Purified RNA is eluted RNase-free water has OD260/OD280 ratios of 1.9-2.1 (measured in 10 mM Tris-Cl, pH 7.5) and is ideal for use in most downstream applicants including Northern blotting, RT-PCR, Quantitative PCR, Poly (A) RNA selection and Array analysis.
Features
- Fast. Using A rapid spin column format, the whole procedure takes less than 20 minutes.
- High Qualtiy of RNA. Purified RNA has an OD260/OD280 ratio of 1.9-2.0.
- Versatile. Suitable for purification of total RNA from Various samples such as bacteria, fungi, animals and some of plants.
- Economic
BS88583 50 Preps | BS88584 100 Preps | BS88586 250 Preps | |
RPE Solution | 12 ml | 24 ml | 60 ml |
Ethanol (96-100%) | 48 ml | 96 ml | 240 ml |
RPE Final Volume | 60 ml | 120 ml | 300 ml |
Salt precipitation in Buffer RLT and DW Solution (concentrate) may occur at low temperature. Redissolve the precipitate by warming the solution at 56oC, then cool back down to room temperature before use.
Care must be taken when working with RNA. It is important to maintain an RNase-free environment starting with RNA sample preparation and continue through purification and analysis. Use RNase free tubes, tips, gels. Wear gloves at all the time
Qualty Control
Each lot of EZ-10 Total RNA Mini-Preps Kit is tested against predetermined specifications to ensure to sonsistent product quality.
Part# | BS88136 |
Decription | EZ-10 DNAaway RNA Miniprep Kit |
Sterile | No |
Storage Conditions | 18 to 25C |
Qty | 1 |
Shipping Conditions | RT |
Size | 250 Preps |
Technical Specifications |
|
---|