Molecular Biology Kits
Storage
The kit is valid for 1 year at 4°C.
Introduction
This kit is designed for preparation of high quality total RNA from a wide variety of plant species and tissues types. Plant tissue are lysed and homogenized by Buffer Rlysis-P. All contaminants, such as polysaccharide, are removed by centrifugation. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation.
Features
- Fast. The whole procedure can be completed in 40 minutes.
- High Quality of RNA. Purified plant total RNA can be used large range of application. Its OD260/OD280 ratio is > 1.8.
- Versatile. Suitable for isolation of total RNA from a wide range of specimens such as arabidopsis thaliana, tobacco, camphor and other samples.
- Easy to scale up.
NOTE: Care must be taken when working with RNA. It is important to maintain an RNAse-free environment starting with RNA sample preparation and continue through purification and analysis. Use RNAse free tubes, tips, gels. Wear gloves at all the time.
Materials Supplied by User:
- Microcentrifuge capable of at least 12,000 × g
- RNase-Free pipets and pipet tips
- Vortexer
- RNase-Free Ethanol (96-100%)
- RNase-Free Microcentrifuge tubes (1.5 ml or 2 ml) Procedures
Components | PT4191 50 Preps |
Buffer Rlysis-P | 35 ml |
Buffer PK | 4 ml |
RNase-free Water | 5 ml |
Protocol | 1 |
Part# | PT4191 |
Decription | Rapid Plant RNA Isolation Kit |
Sterile | No |
Storage Conditions | 18 to 25C |
Qty | 1 |
Shipping Conditions | RT |
Preps | 50 |
Technical Specifications |
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