Molecular Biology Kit, Rapid Bacteria RNA Isolation Kit, 50 Preps

Storage

Store at room temperature. The kit is valid for 1 year at 4°C.

Introduction

This kit is designed for preparation of high quality total RNA from bacteria cells. 20 μg total RNA can be purified from 5 x 107 bacteria cells using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly (A) purification, nuclease protection and in vitro translation. NOTE: Care must be taken when working with RNA. It is important to maintain an RNAse-free environment starting with RNA sample preparation and continue through purification and analysis. Use RNAse free tubes, tips, gels. Wear gloves at all times. 

Features: 

• Fast. Using fast lysis buffer, the whole procedure takes less than 40 minutes.
• High Quality of RNA. Purified RNA has an OD260/OD280 ratio of 1.9-2.0.
• Easy to scale up

Materials Supplied by User:

• Microcentrifuge capable of at least 12,000 × g
• RNase-Free pipets and pipet tips
• Vortexer
• RNase-Free Ethanol (96-100%)
• RNase-Free Microcentrifuge tubes (1.5 ml or 2 ml) Procedures 

Product Info