Molecular Biology Kit, 96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based), 2 Plates

Storage

With the exception of the RNase A, the kit may be stored at room temperature. The RNase A should be stored at 4ºC. The kit is stable for 12 months at room temperature. For longer storage, keep all contents in cold place.

Principle

The EZ-10 96 Well Spin Column Plasmid DNA Purification kit provides a simple, efficient and automated high throughput method for plasmid DNA purifications. Plasmid DNA is selectively adsorbed in silica gel-based EZ-10 columns in the 96 Well Binding Plate and other impurities such as proteins, salts and nucleotides are removed. Plasmid DNA can be eluted in a small volume of Tris buffer.

Note

This kit is used for preparation of up to 10 µg of pure plasmid DNA in each well.

Applications

Plasmid DNA purification

Features

• Fast: Entire procedure takes approximately 45 minutes EZ-10 96 Well Handbook
• High quality: Purified DNA can be used in any downstream applications such as sequencing, transformation, restriction enzymatic digestion, and ligation
• High yield (>80%) and reproducible
• Convenient and environmentally friendly. No phenol/chloroform extraction or ethanol precipitation required

Quality Control

All components in the kit are tested in purification of 96 x 10 µg pUC18 plasmid DNA from overnight culture.

Kit Contents