Molecular Biology Kit, 96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based), 5 Plates
Molecular Biology Kits
Storage
With the exception of the RNase A, the kit may be stored at room temperature. The RNase A should be stored at 4ºC. The kit is stable for 12 months at room temperature. For longer storage, keep all contents in cold place.
Principle
The EZ-10 96 Well Spin Column Plasmid DNA Purification kit provides a simple, efficient and automated high throughput method for plasmid DNA purifications. Plasmid DNA is selectively adsorbed in silica gel-based EZ-10 columns in the 96 Well Binding Plate and other impurities such as proteins, salts and nucleotides are removed. Plasmid DNA can be eluted in a small volume of Tris buffer.
Note
This kit is used for preparation of up to 10 µg of pure plasmid DNA in each well.
Applications
Plasmid DNA purification
Features
- Fast: Entire procedure takes approximately 45 minutes EZ-10 96 Well Handbook
- High quality: Purified DNA can be used in any downstream applications such as sequencing, transformation, restriction enzymatic digestion, and ligation
- High yield (>80%) and reproducible
- Convenient and environmentally friendly. No phenol/chloroform extraction or ethanol precipitation required
Quality Control
All components in the kit are tested in purification of 96 x 10 µg pUC18 plasmid DNA from overnight culture.
Kit Contents
Components | B814152-0002 2 Plates | B814152-0005 5 Plates |
RNase A (10mg/ml) | 0.5ml | 1.2 ml |
Solution 1(A) | 25 ml | 60 ml |
Solution II(B) | 50 ml | 120 ml |
Solution III | 90 ml | 210 ml |
Wash Solution(C) | 2x40 ml | 5x40 ml |
96 Well Filter Plate | 2 | 5 |
EZ-10 96 Well Binding PlateVacuum Manifold Fit | 2 | 5 |
Deep Well Collection Plate | 4 | 10 |
96 Well Storage Plate | 2 | 5 |
Sealing Film | 10 | 25 |
Protocol | 1 | 1 |
Part# | B814152-0005 |
Decription | 96-Well Plate Plasmid DNA Mini-Preps Kit (Vacuum Based) |
Sterile | No |
Storage Conditions | 18 to 25C |
Qty | 1 |
Shipping Conditions | RT |
Qty | 5 Plates |
Technical Specifications |
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