Single Step Ultra Competent Cell Preparation Kit for Preparation of E. Coli Competent Cells for Transformation, 25ml

Description: SSCS is supplied as a ready to use format of 1x solution.

Application: Preparation of E. coli competent cells for transformation.

This Kit contains SSCS solution, enables researchers to prepare competent E. coli cells in a single step and to transform the cells without heatshock. SSCS has been reported to be faster and easier than other methods of producing competent cells, such as the traditional CaCl2 method described by Sambrook et al, or other high competency protocols. The competent cells obtained by this method can be kept at 70°C for long term storage. Transformation efficiencies depend on the strain of E. coli, as well as the nature and quality of the transforming DNA. Typical transformation efficiencies are 10^6 to 10^8 pfu/µg plasmid DNA. For example, the transformation efficiencies observed for E. coli strains DH1, DH5a, HB101, Jm109, Le392, MM294, SCS1, XL1blue and TG1 ranged from 1.5×10^6 to 1.0×10^8 pfu per µg of DNA.

Contents:

Features:

• Simple & Fast. Entire procedure takes about 5 minutes.
• Stable at 70°C with little or no loss in transformation efficiency. It is suitable for long term storage.
• No heat shock required.
• High transformation efficiencies of 10^6 to 10^8 pfu/µg DNA are typically obtained.
• Reproducible.

Quality Control:

Transformation and storage solution is tested for transformation efficiency with appropriate E. coli strains and pUC18 or pUC19 DNA. 

Storage:

All contents of the kit should be kept in freezer after usage. The Kit is stable for 9 months.

Technical Specifications:

Product Info