Molecular Biology Kit, EZ-10 DNAaway RNA Miniprep Kit, 50 Preps

Introduction

EZ-10 Total RNA Mini-Preps Kit allows efficient purification of total RNA from various samples, total RNA is easily purified from animal or human cells and tissues using a simple spin format.

Biological samples are first lysed and homogenized in denaturing guanidine-containing buffer (Buffer RLT), which immediately inactivates DNases and RNases as well as proteases to ensure isolation of intact RNA.  RNA in the whole homogenate is selectively absorbed on EZ-10 Spin Column, all protein and other components are removed in flow-through.  Remaining contaminants amd salts are efficiently washed away.  Purified RNA is eluted RNase-free water has OD₂₆₀/OD₂₈₀ ratios of 1.9-2.1 (measured in 10 mM Tris-Cl, pH 7.5) and is ideal for use in most downstream applicants including Northern blotting, RT-PCR, Quantitative PCR, Poly (A) RNA selection and Array analysis.

Features

• Fast.  Using A rapid spin column format, the whole procedure takes less than 20 minutes.
• High Qualtiy of RNA.  Purified RNA has an OD₂₆₀/OD₂₈₀ ratio of 1.9-2.0.
• Versatile.  Suitable for purification of total RNA from Various samples such as bacteria, fungi, animals and some of plants.
• Economic

Salt precipitation in Buffer RLT and DW Solution (concentrate) may occur at low temperature.  Redissolve the precipitate by warming the solution at 56^oC, then cool back down to room temperature before use.

Care must be taken when working with RNA.  It is important to maintain an RNase-free environment starting with RNA sample preparation and continue through purification and analysis.  Use RNase free tubes, tips, gels.  Wear gloves at all the time

Qualty Control

Each lot of EZ-10 Total RNA Mini-Preps Kit is tested against predetermined specifications to ensure to sonsistent product quality.

Product Info