SeedEZ 3D Cell Culture Scaffold for 24 Well Plates, 24/Pack

SeedEZ 3D Cell Culture Scaffold for 24 Well Plates, 24/Pack

SeedEZ 3D Cell Culture Scaffold for 48 Well Plates, 24/Pack

SeedEZ 3D Cell Culture Scaffold for 48 Well Plates, 24/Pack

SeedEZ 3D Cell Culture Scaffold for 12 Well Plates, 6/Pack

SeedEZ 3D Cell Culture Scaffold for 12 Well Plates, 6/Pack

SeedEZ 3D Cell Culture Scaffold for 24 Well Plates, 24/Pack

Availability: In stock
SKU
SC-C024-0024
Description

SeedEZ 3D Cell Culture Scaffold

$207.00
SeedEZ 3D Cell Culture Scaffold for 24 Well Plates, 24/Pack is available to buy in increments of 1

Our SeedEZ cell culture scaffold allows cells to grow in three dimensions (3D) as in living tissues. The scaffold is composed of glass microfibers that are completely inert and transparent. The arrangement of the microfibers creates tiny pores that allow for the passage of nutrients and gases needed for cell survival. The abundance of room in the scaffold enables the cells to divide, branch out, and take on their natural geometry as if they were still inside the human body.

SeedEZ enables the cells to utilize their cadherin (cell to cell) and integrin (cell to extracellular matrix) receptors which are vital for tissue representative cell behavior, drug responses, and cell therapy outcomes.

SeedEZ is a completely inert, transparent, and hydrophilic glass fiber scaffold that is optimized for long-term cell growth and drug testing. It is straightforward to use and looks like and works like a piece of paper; simply pipet cells in medium, or cells in a hydrogel, and the scaffold will wick them into its interior. Assays may be run in situ, supernatants transferred to another plate for plate reader readouts, and cultures transferred to another dish for imaging.

Examples of in-well assays with quantitative, plate reads, include alamarBlue (cell viability and respiratory metabolism), CYP450glo (activity of drug metabolizing enzymes and drug metabolism), total LDH (live cell number based on standard curve), LDH release to medium (cell death and cytotoxicity) etc. Following an assay, transfer supernatants to another plate and read.

To image cells, use fluorescence microscopy and live/dead cell stains such as Calcein AM and Propidium Iodide.

SeedEZ enables to culture simple and complex culture and co-culture tissue models long-term without worrying that spheroids, or hydrogels comprising cells, will be aspirated in media exchanges or peel off the dish.

SeedEZ is 400 um thick, 90% open, and compatible with all cell types, coatings, and extracellular matrices used in cell culture applications to suit diverse experimental requirements. Available as discs that you can place in 6, 12, 24, 48 and 96-well plates or larger dishes.

 

Features:

  • A simple method to culture cells in three dimensions (as in living tissues) that is as easy as culturing cells in a Petri dish.
  • Routine workflow when handling 3D, tissue-like cultures Use methods, protocols and equipment used for 2D cell cultures.
  • Hassle-free manipulation of 3D cell cultures: Cells will not peel of the dish, as in 2D cultures when they reach confluence. Cultures cannot be aspirated into a pipet tip as with hydrogel-based or hanging-drop-based 3D cultures during media changes
  • Long-term experimentation (at least 4 weeks compared to week-long 2D cultures that develop contact inhibition and cell senscence)
  • Repeat drug dose testing
  • The ability to create complex tissue models for predictive in vitro drug testing

 

Applications:

  • Small molecule drug testing (ADME/Tox)
  • Biopharmaceutical testing
  • Cancer research
  • Academic research

 

Data and examples of use

Data 1:  Superior cellular viability and baseline drug metabolism in human liver cells in SeedEZ

Data 1

 

           

Data 2:  Culturing cells in 3D SeedEZ predicts efficacy of known anti-cancer drugs, such as the leading breast cancer drug Herceptin (Trastuzumab); culturing in 2D does not

Data 2

 

 

Data 3:  Growth of HER2+ breast cancer model with the extracellular matrix in SeedEZ

 

 Data 3

 

 

Technical Specifications:

Model NumberSC-C024-0024
DescriptionSeedEZ 3D cell culture system for 24 well plates (pack of 24 scaffolds)
Qty24 Scaffolds 

 

SeedEZ Downloadable Flyers

More Information
Technical Specifications
Model NumberSC-C024-0024
DescriptionSeedEZ 3D cell culture system for 24 well plates (pack of 24 scaffolds)
Qty24 Scaffolds