BRAND Fluorimetry Cuvettes, UV, 500 Pack

BRAND Fluorimetry Cuvettes, UV, 500 Pack

BRAND Fluorimetry Cuvettes, PS, 500 Pack

BRAND Fluorimetry Cuvettes, PS, 500 Pack

BRAND Macro Cuvettes, PMMA, 500 Pack

BRAND Macro Cuvettes, PMMA, 500 Pack

BRAND Fluorimetry Cuvettes, UV, 500 Pack

Availability: In stock
SKU
759128
Description

Fluorimetry Cuvettes

$498.75
BRAND Fluorimetry Cuvettes, UV, 500 Pack is available to buy in increments of 1

NEW! Disposable macro cuvettes with 4 optically clear sides from BRAND suitable for use in flourescence applications. Available in high quality polystyrene for measurements from 340nm to 800nm, as well as a proprietary polymer with ultra-low autofluorescence for precise measurements in both the visible and UV-range (230-800nm). Cuvettes have a standard 10mm light path for use in all standard fluorimeters. Packaging is 100 per tray in trays made of low-dust, non-scratching polystyrene, and are grouped by manufacturing mold cavity to ensure the lowest variation in extinction coefficient.

BRAND UV-Cuvettes are the disposable alternative to quartz cuvettes for applications that require UVtransparency. Unlike Polystyrene (PS) and Polymethyl methacrylate (PMMA or "acrylic") cuvettes, BRAND UV-Cuvettes are transparent down to 220 nm, so they are suited to molecular biology applications, such as A260/280 purity determinations and other analyses in the UV wavelength range. Besides their UV-transparency, this proprietary plastic is also more resistant to chemical attack than PS or PMMA, and so may bring disposability to applications in which disposable cuvettes were not previously an option because of limited compatibility.

When results count, choose BRAND disposable cuvettes with four optically clear sides for fluorescence applications. Available in high quality polystyrene for measurements from 340nm to 800nm, as well as a proprietary polymer for precise measurements in both the visible and UV-range (230-800nm), allowing for shorter excitation wavelength than have been traditionally possible with disposable cuvettes. This UV-transparent plastic also has virtually no autofluorescence for high signal-to-noise ratios. Cuvettes have a standard 10mm light path for use in all standard fluorimeters.

BRAND fluorescence cuvettes, like all BRAND cuvettes, are made of the highest quality raw materials, precision molded for the best optical clarity, with parallel walls of uniform thickness to optimize transmission and minimize refraction. They are then moldcavity sorted ensuring cuvette-to-cuvette consistency for highly reproducible results. With an industry standard 10mm light path, BRAND 4 sided clear cuvettes fit most common fluorometers. Cuvettes are packaged in trays containing 100 cuvettes. Trays are made of low-dust, non-scratching, expanded polystyrene.

Features:

  • Mold-cavity sorted: Each tray contains cuvettes from a single mold cavity to minimize variability between cuvettes.
  • Manufactured by BRAND: One of the leading manufacturers of premium quality disposable cuvettes for nearly thirty years, BRAND is your assurance of reliability.

 

Frequently Asked Questions

What is the light path of the BRAND cuvettes?  All BRAND cuvettes have a light path of 10mm.
 

What is the difference between the PS and UV-Cuvettes?  

Polystyrene (PS) cuvettes are the least expensive, however they have the lowest chemical resistance, and are only suitable for visible light applications, with a lower wavelength limit of around 340nm. BRAND UV-Cuvettes use a proprietary plastic to allow measurement as low as 230nm, and has resistance to many polar organics as well as many acids and bases. The UV-cuvettes also show no autofluorescence.
 

What does "mold cavity matched" mean?  

Cuvettes are injection molded plastic. No matter how close the tolerances are in the molds, there will be some variance. BRAND cuvettes are sorted during the manufacturing process so that each package of cuvettes contains only cuvettes made from the same mold, minimizing the cuvette-to-cuvette variance.
 

What is the maximum temperature the PS and UV-cuvettes can tolerate?  

Both the PS and UV-cuvettes should not be used at temperatures above 70°C.
 

When should you use quartz cuvettes?  

No plastic is as clear as quartz, as indicated in the transmission curves, below. In certain applications–such as an extremely dilute sample where the utmost in sensitivity is essential–quartz may be the only option, and you'll have to sacrifice the advantages of UV-Cuvettes. With typical samples, however, including DNA and RNA, just use the UV-Cuvette as your "blank" or standard, and the spectrophotometer will adjust for the difference in absorbance between quartz and our UV-plastic. With our free sample packs, you can give the BRAND UV-Cuvettes a try at no cost, and confirm for yourself.
 

About fluorescence measurement:

Fluorescence occurs when energy is supplied to a sample by electromagnetic radiation, usually UV-light (photons). The energy “excites” the molecules thereby “kicking” an electron of an atom from a lower energy state into an excited higher energy state. The lifetime of the excited state is very short (1-100ns). When the electron falls back to a lower energy state it releases the energy in the form of light i.e. fluorescence. Some of the fluorescent light passes through a monochromator and then reaches a detector. The fluorescent light is emitted in all directions, however the detector is usually placed at 90° to the excitation light beam to minimize interference. Hence the need for four-sided clear cuvettes for fluorescence applications.
 
Technical Specifactions:
 
 Material
Filling Vol.
Window
Exterior
Light
Pack of
Cat. No.
Min.
Max.
Dimensions, mmDimensions, mmPath, mm
 Uv
2.5
4.5
10 x 35
12.5x12.5x45
10
500
759128
 

Plastic Cuvette Chemical Compatibility Chart:

ChemicalPSPMMAUV
Acetic acid (96%)PoorPoorExcellent
AcetonePoorPoorExcellent
AcetonitrilePoorPoorExcellent
BenzenePoorPoorPoor
Butanone (methyl ethyl ketone, MEK)PoorPoorExcellent
Carbon TetrachloridePoorPoorPoor
ChloroformPoorPoorPoor
Diethy EtherPoorPoorMarginal
Dimethyl SulfoxideMarginalPoorExcellent
EthanolPoorPoorExcellent
Formaldehye (40%)PoorPoorExcellent
HeptanePoorMarginalPoor
HexanePoorExcellentPoor
Hydrochloric Acid (32%)MarginalPoorExcellent
IsopropanolMarginalMarginalExcellent
n-PentanePoorPoorPoor
Oleic AcidPoorPoorPoor
Perchloric Acid (10%)PoorPoorExcellent
Petroleum EtherPoorPoorMarginal
PhenolPoorPoorExcellent
Sodium HydroxideExcellentPoorExcellent
Sulfuric AcidPoorPoorExcellent
Trichloroacetic AcidPoorPoorExcellent
TrichloroethylenePoorPoorPoor
ToluenePoorPoorPoor

 

Excellent: Properties of BRAND Disposable Cuvettes remain intact after 24 hours of exposure.
Marginal: Properties of BRAND Disposable Cuvettes may not remain intact after 24 hours of exposure. Testing is recommended prior to use.
Poor: Properties of BRAND Disposable Cuvettes will not remain intact after 24 hours of exposure.

 
More Information
Technical Specifications
 Material
Filling Vol.
Window
Exterior
Light
Pack of
Cat. No.
Min.
Max.
Dimensions, mmDimensions, mmPath, mm
 UV
2.5
4.5
10 x 35
12.5x12.5x45
10
500
759128